Higashi H, Hirabayashi Y, Fukui Y, Naiki M, Matsumoto M, Ueda S, Kato S
Cancer Res. 1985 Aug;45(8):3796-802.
Hanganutziu-Deicher (HD) antigen-active N-glycolylneuraminic acid (NeuGc)-containing gangliosides were isolated and characterized from human colon cancer tissues. The antigenic gangliosides were detected by thin-layer chromatography by our newly developed method (H. Higashi, Y. Fukui, S. Ueda, S. Kato, Y. Hirabayashi, M. Matsumoto, and M. Naiki. J. Biochem., 95: 1517-1520, 1984) of enzyme immunostaining using affinity-purified chicken antibody against hematoside containing NeuGc (II3NeuGc-LacCer) and horseradish peroxidase-conjugated rabbit anti-chicken lgG. One to six species of the antigenic gangliosides were isolated from seven of 16 cases of colon cancer, whereas no antigenic compound was detected in all apparently normal colorectal tissues from 17 individuals without colorectal cancer. Tissues from different patients showed different patterns of molecular species of the antigenic gangliosides. Densitometric determination indicated that HD antigenic sialic acid, NeuGc, accounted for about 1% or less of the total lipid-bound sialic acids. Four species of antigenic gangliosides were identified as hematoside and hematoside-containing O-acyl ester (II3NeuGc-LacCer and II3 4- or 7-O-acyl-NeuGc-LacCer), GM2-containing NeuGc (II3NeuGc-GgOse3Cer), and sialylparagloboside (IV3NeuGc-nLcOse4Cer) by their behaviors on 2-dimensional thin-layer chromatography, and the effects of mild alkaline treatment, sialidase treatment, periodate oxidation, and endo-beta-galactosidase treatment.
从人结肠癌组织中分离并鉴定了含有Hanganutziu-Deicher（HD）抗原活性的N-羟乙酰神经氨酸（NeuGc）的神经节苷脂。采用我们新开发的方法（H. Higashi、Y. Fukui、S. Ueda、S. Kato、Y. Hirabayashi、M. Matsumoto和M. Naiki。《生物化学杂志》，95: 1517 - 1520，1984年），即使用针对含NeuGc的血苷（II3NeuGc-LacCer）的亲和纯化鸡抗体和辣根过氧化物酶偶联的兔抗鸡IgG进行酶免疫染色的薄层色谱法，检测抗原性神经节苷脂。从16例结肠癌患者中的7例分离出1至6种抗原性神经节苷脂，而在17例无结直肠癌的个体的所有明显正常的结直肠组织中均未检测到抗原性化合物。来自不同患者的组织显示出抗原性神经节苷脂分子种类的不同模式。光密度测定表明，HD抗原性唾液酸NeuGc占总脂质结合唾液酸的约1%或更少。通过二维薄层色谱行为以及温和碱性处理、唾液酸酶处理、高碘酸盐氧化和内切β-半乳糖苷酶处理的效果，鉴定出4种抗原性神经节苷脂为血苷和含血苷的O-酰基酯（II3NeuGc-LacCer和II3 4-或7-O-酰基-NeuGc-LacCer）、含NeuGc的GM2（II3NeuGc-GgOse3Cer）和唾液酰副球蛋白苷（IV3NeuGc-nLcOse4Cer）。
