Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine, St. Louis, Missouri, USA.
Genome Technology Access Center, Washington University School of Medicine, St. Louis, Missouri, USA.
FASEB J. 2019 Jul;33(7):8386-8405. doi: 10.1096/fj.201802281R. Epub 2019 Apr 16.
Periostin (), a secretory matricellular matrix protein, plays a multitude of biologic functions. Various splice variants of have been described; however, their expression pattern and functional implications are not completely understood. This study was undertaken to decipher the differential expression pattern of and its splice variants in various tissues and cell types. We show that was more highly expressed in anterior cruciate ligament (ACL) remnants compared with articular cartilage at the cellular and tissue level. Isoforms 1 and 8 were highly expressed only in articular chondrocytes, suggesting their splice-specific regulation in chondrocytes. To discern the role of total and full-length human POSTN isoform 1 (), we stably transfected human chondrosarcoma 1 (hCh-1) cell line with using a pcDNA3.1-hPOSTN-001 construct. RNA-sequencing analysis of hCh-1 cells identified differentially expressed genes with a known role in chondrocyte function and osteoarthritis. Similar expression of a subset of candidate genes was revealed in ACL progenitor cells and chondrocytes as well as in ACL progenitor cells in which activity was altered by overexpression and by small interfering RNA gene knockdown. Cells expressing total , not isoform 1, exhibited increased cell adhesion potential. These findings suggest an important role for in the knee.-Cai, L., Brophy, R. H., Tycksen, E. D., Duan, X., Nunley, R. M., Rai, M. F. Distinct expression pattern of periostin splice variants in chondrocytes and ligament progenitor cells.
纤调蛋白（）是一种分泌型细胞外基质蛋白，具有多种生物学功能。已经描述了的各种剪接变体；然而，它们的表达模式和功能意义尚不完全清楚。本研究旨在破译在各种组织和细胞类型中纤调蛋白及其剪接变体的差异表达模式。我们表明，在细胞和组织水平上，前交叉韧带（ACL）残端中表达的高于关节软骨。同工型 1 和 8 仅在关节软骨细胞中高度表达，表明它们在软骨细胞中的剪接特异性调节。为了辨别总和全长人 POSTN 同工型 1（）的作用，我们使用 pcDNA3.1-hPOSTN-001 构建体将稳定转染人软骨肉瘤 1（hCh-1）细胞系的。hCh-1 细胞的 RNA 测序分析鉴定了具有已知在软骨细胞功能和骨关节炎中作用的差异表达基因。在 ACL 祖细胞和软骨细胞以及通过过表达和小干扰 RNA 基因敲低改变的 ACL 祖细胞中，也揭示了候选基因子集的相似表达。表达全长的细胞，而不是同工型 1，表现出增加的细胞粘附潜力。这些发现表明在膝关节中，纤调蛋白在 ACL 祖细胞和软骨细胞中高度表达，在 ACL 祖细胞和软骨细胞中高度表达。
