Biotechnology, Institute of Tropical Pathology and Public Health, Universidade Federal de Goiás, Goiânia, GO 74605-050, Brazil.
Chemical Institute, Universidade Federal de Goiás, Goiânia, GO 74001-970, Brazil.
Bioorg Med Chem. 2019 Jun 15;27(12):2637-2643. doi: 10.1016/j.bmc.2019.04.011. Epub 2019 Apr 6.
Drug delivery systems prepared with nanostructures are able to overcome biological barriers. However, one of the main challenges in the use of these nanosystems is their internalization by macrophages. This study aims to prepare and characterize chitosan nanoparticles incorporating maghemite nanoparticles and investigate their intracellular tracking in RAW 264.7 macrophages in vitro. Then, maghemite nanoparticles were encapsulated within chitosan nanoparticles by ionotropic gelification method. The images from transmission electron microscopy were used to investigate the intracellular penetration of conjugated nanoparticles by macrophages using different times. Our data suggests that magnetic nanoparticles are suitable to act as a contrast agent to investigate the cellular internalization of chitosan nanoparticles.
用纳米结构制备的药物传递系统能够克服生物屏障。然而，这些纳米系统应用的主要挑战之一是巨噬细胞的内化。本研究旨在制备并表征载有磁赤铁矿纳米颗粒的壳聚糖纳米粒子，并研究其在 RAW 264.7 巨噬细胞中的细胞内跟踪情况。然后，采用离子凝胶化法将磁赤铁矿纳米颗粒包封在壳聚糖纳米粒子中。通过透射电子显微镜图像，研究了不同时间巨噬细胞内共轭纳米粒子的穿透情况。我们的数据表明，磁性纳米颗粒适合作为对比剂，用于研究壳聚糖纳米粒子的细胞内化。
