Department of Pediatrics, Linyi Central Hospital, Linyi, Shandong 276400, China.
Department of Surgery, Maternal and Child Health Care Family Planning Service Center of Yishui County, Linyi, Shandong 276400, China
Biosci Rep. 2019 May 17;39(5). doi: 10.1042/BSR20190602. Print 2019 May 31.
LncRNA SOX21 antisense RNA 1 (SOX21-AS1) dysregulated in many types of human cancer, and functioned as tumor suppressor or promoter depending on tumor types. However, there was no report about the role of SOX21-AS1 in nephroblastoma. In the present study, we first found that SOX21-AS1 expression was elevated in nephroblastoma tissues and cell lines compared with adjacent normal tissues and normal human embryonic kidney cell line, respectively. Moreover, we observed nephroblastoma patients with large tumor size, advanced National Wilms Tumor Study (NWTS) stage or unfavorable histopathological type, and patients that had higher SOX21-AS1 expression levels than nephroblastoma patients with small tumor size, early NWTS stage or favorable histopathological type. The studies suggested that knockdown of SOX21-AS1 suppressed nephroblastoma cell proliferation and colony formation, and induced cell-cycle arrest through up-regulating p57 expression. In conclusion, our study suggests that SOX21-AS1 functions as oncogenic lncRNA in nephroblastoma, which may provide a novel insight for nephroblastoma carcinogenesis.
长链非编码 RNA SOX21 反义 RNA 1（SOX21-AS1）在许多人类癌症中失调，其功能取决于肿瘤类型，可以作为肿瘤抑制因子或促进因子。然而，目前尚无关于 SOX21-AS1 在肾母细胞瘤中的作用的报道。在本研究中，我们首先发现与相邻正常组织和正常人胚肾细胞系相比，SOX21-AS1 在肾母细胞瘤组织和细胞系中的表达升高。此外，我们观察到肿瘤体积较大、国家威尔姆斯肿瘤研究（NWTS）分期较晚或组织病理学类型较差的肾母细胞瘤患者，以及 SOX21-AS1 表达水平高于肿瘤体积较小、NWTS 分期较早或组织病理学类型较好的肾母细胞瘤患者。这些研究表明，下调 SOX21-AS1 通过上调 p57 的表达抑制肾母细胞瘤细胞的增殖和集落形成，并诱导细胞周期停滞。总之，我们的研究表明 SOX21-AS1 在肾母细胞瘤中作为致癌 lncRNA 发挥作用，这可能为肾母细胞瘤的发生提供新的见解。
