Henan Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Key Laboratory of Green Chemical Media and Reactions, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering Institution, Henan Normal University, Xinxiang 453007, China.
Chem Commun (Camb). 2019 Apr 30;55(36):5279-5282. doi: 10.1039/c9cc01451a.
Under the synergistic effects of protonation and deprotonation, a light-activated fluorescent probe (UV-SP) exhibited "cycle-reversible intramolecular charge transfer (ICT)" for different pH after activation by UV light, resulting in emission of multiple ratio fluorescent signals (FI563/FI595 and FI664/FI595). Based on these kinds of response signals, UV-SP can specifically monitor the cycle-reversible trace change of intracellular pH caused by UV radiation. More importantly, according to the stable and invariant multiple ratio fluorescent signals, UV-SP can sort cells entering programmed death.
在质子化和去质子化的协同作用下，一种光激活荧光探针（UV-SP）在紫外光激活后，针对不同 pH 值表现出“循环可逆分子内电荷转移（ICT）”，从而发出多种比率荧光信号（FI563/FI595 和 FI664/FI595）。基于这些响应信号，UV-SP 可以特异性监测由紫外辐射引起的细胞内 pH 值的循环可逆痕量变化。更重要的是，根据稳定且不变的多比率荧光信号，UV-SP 可以对进入程序性死亡的细胞进行分类。
