Ren Mingyao, Chen Zhe, Ge Chuandong, Hu Wei, Xu Jing, Yang Limin, Luan Mingming, Wang Nianxing
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China.
Pharmaceutics. 2022 Jun 25;14(7):1349. doi: 10.3390/pharmaceutics14071349.
MiRNA-targeted therapy is an active research field in precision cancer therapy. Studying the effect of miRNA expression changes on apoptosis is important for evaluating miRNA-targeted therapy and realizing personalized precision therapy for cancer patients. Here, a new fluorescent nanoprobe was designed for the simultaneous imaging of miRNA-21 and apoptotic protein caspase-3 in cancer cells by using gold nanoparticles as the core and polydopamine as the shell. Confocal imaging indicated that the nanoprobe could be successfully applied for in situ monitoring of miRNA regulation of apoptosis. This design strategy is critical for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA, and developing personalized treatment plans.
微小RNA靶向治疗是精准癌症治疗中一个活跃的研究领域。研究微小RNA表达变化对细胞凋亡的影响，对于评估微小RNA靶向治疗以及实现癌症患者的个性化精准治疗具有重要意义。在此，设计了一种新型荧光纳米探针，以金纳米颗粒为核心、聚多巴胺为外壳，用于同时对癌细胞中的微小RNA-21和凋亡蛋白半胱天冬酶-3进行成像。共聚焦成像表明，该纳米探针可成功应用于原位监测微小RNA对细胞凋亡的调控。这种设计策略对于研究微小RNA靶向治疗的可行性、筛选靶向微小RNA的新型抗癌药物以及制定个性化治疗方案至关重要。
