Ikossi M G, Ambrus J L, Chadha K C
Res Commun Chem Pathol Pharmacol. 1986 Dec;54(3):379-93.
A great variability exists in the in vitro capability of peripheral blood leukocytes (PBL) of apparently healthy adults to produce IFN-alpha. In this report, we have studied the effects of prostaglandins, indomethacin and pentoxifylline upon IFN-alpha production by the PBL of normal healthy donors. The donors were divided into two groups: "normal producers" - donors that consistently produced greater than 2,000 ref. units IFN-alpha per 10(7) PBL and "low producers" - donors that consistently produced less than 2,000 ref. units IFN-alpha per 10(7) PBL. Addition of exogenous PGE1 or PGE2 into interferon production media significantly inhibited IFN-alpha yield by the PBL of both normal and low producers. The inhibition was dose dependent. A significant rise in the IFN-alpha yield was seen when PBL of "low producers" were exposed individually to either pentoxifylline or indomethacin. However, no increase in titer of IFN-alpha was seen when PBL of "normal producers" were exposed to either of these two agents during interferon production periods. With regard to PBL of "low producers", the enhancement of IFN-alpha yield was further potentiated when both indomethacin and pentoxifylline were utilized concurrently at their optimal concentrations. These results suggest that enhancement of IFN-alpha production by PBL of apparently healthy adults who are low producers may be possible and may have preventive value.
表面健康的成年人外周血白细胞（PBL）产生干扰素-α（IFN-α）的体外能力存在很大差异。在本报告中，我们研究了前列腺素、吲哚美辛和己酮可可碱对正常健康供体PBL产生IFN-α的影响。供体被分为两组：“正常产生者”——每10⁷个PBL持续产生超过2000参考单位IFN-α的供体，以及“低产生者”——每10⁷个PBL持续产生少于2000参考单位IFN-α的供体。在干扰素生产培养基中添加外源性前列腺素E1（PGE1）或前列腺素E2（PGE2）可显著抑制正常产生者和低产生者PBL产生IFN-α的产量。这种抑制作用呈剂量依赖性。当“低产生者”的PBL分别暴露于己酮可可碱或吲哚美辛时，IFN-α产量显著增加。然而，在干扰素生产期间，当“正常产生者”的PBL暴露于这两种药物中的任何一种时，IFN-α滴度均未增加。对于“低产生者”的PBL，当吲哚美辛和己酮可可碱同时以最佳浓度使用时，IFN-α产量的提高会进一步增强。这些结果表明，对于低产生者的表面健康成年人，其PBL产生IFN-α的能力有可能得到增强，且可能具有预防价值。
