Barros Bárbara R S, Barboza Bruno Rafael, Ramos Bárbara A, Moura Maiara C DE, Coelho Luana C B B, Napoleão Thiago Henrique, Correia Maria Tereza S, Paiva Patrícia Maria G, Cruz Filho Iranildo José DA, Silva Túlio Diego DA, Lima Cláudia S A, Melo Cristiane M L DE
Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Artur de Sá, s/n, 50740-520 Recife, PE, Brazil.
Department of Biochemistry, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, PE, Brazil.
An Acad Bras Cienc. 2019;91(1):e20190916. doi: 10.1590/0001-3765201920180358. Epub 2019 Apr 8.
Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPLC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were evaluated by cytometry. We found a list of phenolic compounds among other bioactive compounds in the M. emarginata saline extract. In addition, higher antioxidant profile and antifungal activity against different strains of Candida spp. was promoted by the saline extract. Splenocytes showed greater cell viability (more than 90%) and showed higher proliferate index in 24 and 48 hours of incubation with the extract. Saline extract from Malpighia emarginata DC has potential action like antioxidant and antifungal agent without promote animal cell damage.
目前，对具有生物活性的新型天然化合物的研究显示出巨大的民族药理学重要性。在本研究中，我们评估了大果黄褥花（Malpighia emarginata DC）叶的盐水提取物所具有的生物学特性，其目的是评估抗氧化、抗菌和细胞毒性潜力。通过超高效液相色谱 - 质谱联用（UPLC-MS）色谱法进行植物化学特征分析以鉴定化合物。对于抗氧化潜力，使用了1,1-二苯基-2-三硝基苯肼（DPPH）、总抗氧化能力（ATT）和铁离子还原抗氧化能力（FRAP）方法。抗菌和抗真菌试验通过评估半数抑制浓度50（MIC50）、半数抑制浓度90（MIC90）、杀菌浓度（CMB）和杀真菌浓度（CMF）参数来进行。此外，还评估了抗生物膜作用。使用来自Balb/c小鼠的脾细胞进行细胞毒性和增殖实验，并通过细胞计数法进行评估。我们在大果黄褥花盐水提取物中发现了一系列酚类化合物以及其他生物活性化合物。此外，该盐水提取物具有更高的抗氧化活性以及对不同念珠菌属菌株的抗真菌活性。脾细胞在与提取物孵育24小时和48小时后显示出更高的细胞活力（超过90%）和更高的增殖指数。大果黄褥花（Malpighia emarginata DC）的盐水提取物具有作为抗氧化剂和抗真菌剂的潜在作用，且不会对动物细胞造成损伤。
