Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clinica Infantil, Ribeirão Preto, São Paulo, Brasil.
Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Materiais Dentários e Prótese, Ribeirão Preto, São Paulo, Brasil.
J Appl Oral Sci. 2019;27:e20180426. doi: 10.1590/1678-7757-2018-0426. Epub 2019 Apr 11.
Enamel demineralization is among the main topics of interest in the orthodontic field. Self-ligating brackets have been regarded as advantageous in this aspect. The aim of this study was to evaluate the break homeostasis in the oral environment and the levels of microorganisms associated with dental caries among the different types of brackets.
Twenty patients received two self-ligating brackets: In-Ovation®R, SmartClipTM, and one conventional GeminiTM. Saliva was collected before bonding (S0), 30 (S1) and 60 (S2) days after bonding. One sample of each bracket was removed at 30 and 60 days for the in situ analysis. Checkerboard DNA-DNA Hybridization was employed to evaluate the levels of microbial species as-sociated with dental caries. Data were evaluated by nonparametric Friedman and Wilcoxon tests at 5% significance level.
The salivary levels of L. casei (p=0.033), S. sobrinus (p=0.011), and S. sanguinis (p=0.004) increased in S1. The in situ analyses showed alteration in S. mutans (p=0.047), whose highest levels were observed to the In-Ovation®R.
The orthodontic appliances break the salivary homeostasis of microorganisms involved in dental caries. The contamination pattern was different between self-ligating and conventional brackets. The In-Ovation®R presented worse performance considering the levels of cariogenic bacterial species.
釉质脱矿是正畸领域的主要关注点之一。自锁托槽在这方面被认为是有利的。本研究旨在评估不同类型托槽在口腔环境中的断流平衡和与龋齿相关的微生物水平。
20 名患者接受了两种自锁托槽：In-Ovation®R、SmartClipTM 和一种传统的 GeminiTM。在粘接前（S0）、粘接后 30 天（S1）和 60 天（S2）收集唾液。在第 30 天和第 60 天，每个托槽各取一个样本进行原位分析。采用斑点杂交 DNA-DNA 杂交技术评估与龋齿相关的微生物种属水平。数据采用非参数 Friedman 和 Wilcoxon 检验在 5%的显著性水平进行评估。
S1 时唾液中 L. casei（p=0.033）、S. sobrinus（p=0.011）和 S. sanguinis（p=0.004）的水平升高。原位分析显示 S. mutans（p=0.047）发生改变，In-Ovation®R 托槽的水平最高。
正畸矫治器破坏了与龋齿有关的微生物的唾液稳态。自锁托槽和传统托槽的污染模式不同。In-Ovation®R 托槽在致龋细菌种属水平上的表现较差。
