Department of Pediatric Clinic, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida do Café, S/N, Monte Alegre, Ribeirão Preto, SP, CEP: 14040-904, Brazil.
Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, SP, Brazil.
Clin Oral Investig. 2017 May;21(4):1037-1046. doi: 10.1007/s00784-016-1865-5. Epub 2016 Jun 6.
The aims were to evaluate the levels of bacterial species in saliva and in situ and to assess whether the design of brackets influences the risk of developing periodontal disease.
Twenty patients (13.3 mean age) were bonded with self-ligating brackets and a conventional bracket. Saliva was collected before bonding and 30 and 60 days after bonding. One sample of each bracket was removed 30 and 60 days after bonding. The analysis was determined by checkerboard DNA-DNA hybridization. The data was evaluated by the non-parametric test.
A significant increase in the levels of bacterial species in the saliva occurred in 15 of the 22 analyzed species. The self-ligating brackets presented the highest incidence percentages for the orange and red complexes 60 days after bonding. In situ analyses showed different patterns according to the bracket design. The levels of Campylobacter rectus showed significant differences (p = 0.011) 60 days after bonding among the three brackets; the highest values were observed in the In-Ovation®R bracket.
The bracket design seems to influence the levels of bacterial species involved in periodontal disease. Considering the wide variety of bacterial species, additional studies are needed to aid in the establishment of effective protocols to prevent the development of periodontal disease during orthodontic treatment.
A dynamic alteration in the oral microbiota may lead to inflammatory reactions in the supporting soft and hard tissues. The different types of brackets interfere with bacterial adherence. Bracket design should be considered in orthodontic treatment.
评估唾液中和原位细菌种类的水平，并评估托槽的设计是否会影响牙周病的发病风险。
将 20 名患者（平均年龄 13.3 岁）用自锁托槽和传统托槽粘接。在粘接前和粘接后 30 天和 60 天收集唾液。在粘接后 30 天和 60 天分别取出每个托槽的一个样本。通过斑点杂交 DNA-DNA 杂交分析。用非参数检验评估数据。
在 22 种分析的细菌种中，有 15 种的细菌水平显著增加。自锁托槽在粘接后 60 天呈现出橙色和红色复合体的最高发生率。原位分析显示，根据托槽的设计呈现出不同的模式。在三种托槽中，直肠弯曲杆菌的水平在粘接后 60 天有显著差异（p=0.011）；在 In-Ovation®R 托槽中观察到的数值最高。
托槽的设计似乎影响牙周病相关细菌种类的水平。考虑到细菌种类的多样性，需要进一步研究以帮助建立有效的预防正畸治疗期间牙周病发生的方案。
口腔微生物群的动态变化可能导致支持软硬组织的炎症反应。不同类型的托槽干扰细菌的黏附。在正畸治疗中应考虑托槽的设计。
