Wu Xue-Liang, Wang Yuan-Yuan, Wang Li-Kun, Xue Jun, Yang Dong-Dong, Qu Ming, Wang Chen-Yu, Guo Fei, Yang Rui-Min, Liu Bo
Department of General Surgery, First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China.
Department of Gastrointestinal Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, 050031, China.
Rev Assoc Med Bras (1992). 2019 Mar;65(3):404-409. doi: 10.1590/1806-9282.65.3.404. Epub 2019 Apr 11.
This study aims to investigate the expression of Id-1 in human colorectal adenocarcinoma tissues and explore its correlation with the clinical pathological parameters of colorectal cancer.
The Id-1 mRNA and protein expression levels of 50 specimens of normal colorectal tissues and 50 specimens of colorectal adenocarcinoma tissues were detected using reverse-transcription polymerase chain reaction and western blot. Furthermore, Id-1 protein was detected using immunohistochemistry. The correlation between the expression of Id-1 and clinicopathologic features was analyzed.
The mRNA expression level of Id-1 in colorectal adenocarcinoma tissues and normal colorectal tissues was 0.96 ± 0.03 vs. 0.20 ± 0.04, respectively; and the difference was statistically significant (P=0.011). Furthermore, Id-1 protein expression was higher in colorectal adenocarcinoma tissues than in normal colorectal tissues (0.82 ± 0.04 vs. 0.31 ± 0.02, P=0.020). In addition, the positive protein expression rate of Id-1 was higher in colorectal adenocarcinoma tissues than in normal colorectal tissues (72.00% vs. 24.00%, X2=23.431, P=0.000). The expression of Id-1 was correlated with the depth of tumor invasion, TNM stage, lymph node metastasis, vessel invasion, and liver metastasis (P<0.01). However, this expression was not correlated with tumor size and differentiation degrees (P>0.05).
The high Id-1 expression in colorectal adenocarcinoma tissues play an important role in the process of cancer, and is expected to become a new tumor monitoring indicator for clinical diagnosis, treatment, and prognosis judgment.
本研究旨在探讨Id-1在人大肠腺癌组织中的表达情况，并探讨其与大肠癌临床病理参数的相关性。
采用逆转录聚合酶链反应和蛋白质免疫印迹法检测50例正常大肠组织和50例大肠腺癌组织中Id-1的mRNA和蛋白表达水平。此外，采用免疫组织化学法检测Id-1蛋白。分析Id-1表达与临床病理特征之间的相关性。
大肠腺癌组织中Id-1的mRNA表达水平为0.96±0.03，正常大肠组织中为0.20±0.04；差异具有统计学意义（P=0.011）。此外，Id-1蛋白在大肠腺癌组织中的表达高于正常大肠组织（0.82±0.04 vs. 0.31±0.02，P=0.020）。另外，Id-1蛋白阳性表达率在大肠腺癌组织中高于正常大肠组织（72.00% vs. 24.00%，X2=23.431，P=0.000）。Id-1的表达与肿瘤浸润深度、TNM分期、淋巴结转移、血管侵犯及肝转移相关（P<0.01）。然而，该表达与肿瘤大小及分化程度无关（P>0.05）。
Id-1在大肠腺癌组织中的高表达在肿瘤发生过程中起重要作用，有望成为临床诊断、治疗及预后判断的新型肿瘤监测指标。
