Gil I, Pérez Silva G, del Mar Melgar M
Zentralbl Mikrobiol. 1986;141(6):453-60.
Molecules of polysaccharide, lipopolysaccharide, and peptidoglycane from Pseudomonas aeruginosa were assayed as immunogens and immunomodifiers. The kinetics of humoral responses, phagocytic activity, splenic index variations, and immunomodulating ability of these molecules were studied. Their protective and immunomodifying effects were compared to those similarly produced by heat-killed bacteria suspensions. The influence of the nature and doses of these molecules as well as the intervals between their administration and the SRBC immunization were also studied. Results showed that higher IgM and IgG levels were produced by lower doses of lipopolysaccharide and peptidoglycane, respectively. Using these amphiphilic molecules at doses of 160 micrograms/mouse, a variable immunodepressive activity was observed. This dose-dependent effect which particularly altered the IgM synthesis, was also observed in phagocytic responses. The exopolysaccharide showed to be the most immunodepressive molecule, and this activity appeared to be dependent of dose and time of administration.
对来自铜绿假单胞菌的多糖、脂多糖和肽聚糖分子作为免疫原和免疫调节剂进行了测定。研究了这些分子的体液反应动力学、吞噬活性、脾脏指数变化以及免疫调节能力。将它们的保护和免疫调节作用与热灭活细菌悬液产生的类似作用进行了比较。还研究了这些分子的性质和剂量以及它们给药与绵羊红细胞免疫之间的间隔时间的影响。结果表明，较低剂量的脂多糖和肽聚糖分别产生了较高水平的IgM和IgG。以160微克/小鼠的剂量使用这些两亲性分子时，观察到了可变的免疫抑制活性。这种剂量依赖性效应尤其改变了IgM的合成，在吞噬反应中也观察到了这种效应。胞外多糖被证明是最具免疫抑制作用的分子，并且这种活性似乎取决于给药剂量和时间。
