Palazzolo Stefano, Hadla Mohamad, Spena Concetta Russo, Bayda Samer, Kumar Vinit, Lo Re Francesco, Adeel Muhammad, Caligiuri Isabella, Romano Flavio, Corona Giuseppe, Canzonieri Vincenzo, Toffoli Giuseppe, Rizzolio Flavio
Clinical and Experimental Pharmacology Unit, IRCCS CRO Aviano-National Cancer Institute, 33081 Aviano, Italy.
Doctoral School in Nanotechnology, University of Trieste, 34127 Trieste, Italy.
ACS Med Chem Lett. 2019 Jan 31;10(4):517-521. doi: 10.1021/acsmedchemlett.8b00557. eCollection 2019 Apr 11.
One of the most promising applications of DNA origami is its use as an excellent evolution of nanostructured intelligent systems for drug delivery, but short lifetime and immune-activation are still major challenges to overcome. On the contrary, stealth liposomes have long-circulation time and are well tolerated by the immune system. To overcome DNA origami limitations, we have designed and synthesized a compact short tube DNA origami (STDO) of approximately 30 nm in length and 10 nm in width. These STDO are highly stable ≥48 h in physiological conditions without any postsynthetic modifications. The compact size of STDO precisely fits inside a stealthy liposome of about 150 nm and could efficiently remotely load doxorubicin in liposomes (LSTDO) without a pH driven gradient. We demonstrated that this innovative drug delivery system (DDS) has an optimal tumoral release and high biocompatible profiles opening up new horizons to encapsulate many other hydrophobic drugs.
DNA折纸术最有前景的应用之一是作为纳米结构智能药物递送系统的卓越发展，但短寿命和免疫激活仍是有待克服的主要挑战。相反，隐形脂质体具有长循环时间且免疫系统对其耐受性良好。为克服DNA折纸术的局限性，我们设计并合成了一种紧凑的短管DNA折纸术（STDO），其长度约为30纳米，宽度为10纳米。这些STDO在生理条件下高度稳定，≥48小时，无需任何合成后修饰。STDO的紧凑尺寸恰好能装入约150纳米的隐形脂质体中，并且无需pH驱动梯度就能有效地将阿霉素远程负载到脂质体中（LSTDO）。我们证明，这种创新的药物递送系统（DDS）具有最佳的肿瘤释放和高生物相容性，为封装许多其他疏水药物开辟了新前景。
