Thongpan Ilada, Suntronwong Nungruthai, Vichaiwattana Preeyaporn, Wanlapakorn Nasamon, Vongpunsawad Sompong, Poovorawan Yong
Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
PeerJ. 2019 Apr 11;7:e6748. doi: 10.7717/peerj.6748. eCollection 2019.
Children and adults residing in densely populated urban centers around the world are at risk of seasonal influenza-like illness caused by respiratory viruses such as influenza virus, human metapneumovirus (hMPV), and respiratory syncytial virus (RSV). In a large metropolitan of Thailand's capital city Bangkok, most respiratory infections are rarely confirmed by molecular diagnostics. We therefore examined the frequency of RSV, hMPV, and influenza virus in 8,842 patients who presented influenza-like illness and sought medical care at a large hospital in Bangkok between 2016 and 2017. Using a multiplex real-time reverse-transcription polymerase chain reaction (RT-PCR), 30.5% (2,699/8,842) of nasopharyngeal (NP) swab samples tested positive for one or more of these viruses. Influenza virus comprised 17.3% (1,528/8,842), of which the majority were influenza A/H3N2. Such infection was most prevalent among adults and the elderly. RSV was identified in 11.4% (1,011/8,842) and were mostly ON1 and BA9 genotypes. Of the hMPV-positive samples (3.6%, 318/8,842), genotypes A2, B1, and B2 were detected. A small number of individuals experienced co-infections (1.8%, 155/8,842), most commonly between RSV and influenza A/H3N2. RSV and hMPV co-infections were also found, but mainly in young children. Viral respiratory tract infection peaked locally in the rainy season (June to September). These findings support the utility of rapid nucleic acid testing of RSV, hMPV, and influenza virus in patients with ILI.
居住在世界各地人口密集的城市中心的儿童和成人，面临着由流感病毒、人偏肺病毒（hMPV）和呼吸道合胞病毒（RSV）等呼吸道病毒引起的季节性流感样疾病的风险。在泰国首都曼谷的一个大都市，大多数呼吸道感染很少通过分子诊断得到确诊。因此，我们调查了2016年至2017年间在曼谷一家大型医院就诊的8842例出现流感样疾病并寻求医疗护理的患者中RSV、hMPV和流感病毒的感染频率。使用多重实时逆转录聚合酶链反应（RT-PCR），30.5%（2699/8842）的鼻咽（NP）拭子样本检测出一种或多种这些病毒呈阳性。流感病毒占17.3%（1528/8842），其中大多数是甲型H3N2流感。这种感染在成年人和老年人中最为普遍。RSV在11.4%（1011/8842）中被鉴定出来，主要是ON1和BA9基因型。在hMPV阳性样本（3.6%，318/8842）中，检测到A2、B1和B2基因型。少数个体经历了合并感染（1.8%，155/8842），最常见的是RSV和甲型H3N2流感之间的合并感染。也发现了RSV和hMPV的合并感染，但主要发生在幼儿中。病毒性呼吸道感染在雨季（6月至9月）达到当地高峰。这些发现支持了对流感样疾病患者进行RSV、hMPV和流感病毒快速核酸检测的实用性。
