基于逆转录重组酶聚合酶扩增（RT-RPA）和CRISPR-Cas12a的呼吸道合胞病毒检测
Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a.
作者信息
Khamwut Ariya, Nimnual Juthamas, Chomta Nantinee, Nimsamer Pattaraporn, Mayuramart Oraphan, Kaewsapsak Pornchai, Pasittungkul Siripat, Poovorawan Yong, Payungporn Sunchai
机构信息
Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
出版信息
Exp Biol Med (Maywood). 2025 May 1;250:10387. doi: 10.3389/ebm.2025.10387. eCollection 2025.
Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 10 and 10 copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.
人呼吸道合胞病毒（hRSV）是全球感染儿童最普遍的病毒之一。在本研究中，我们采用了带有CRISPR/Cas12a检测方法的逆转录重组酶聚合酶扩增技术（RT-RPA）来检测和区分A组呼吸道合胞病毒（RSV-A）和B组呼吸道合胞病毒（RSV-B），特别是在资源有限的环境中。RSV-A和RSV-B的检测限分别约为10拷贝/反应和10拷贝/反应。该检测方法在检测RSV-A和RSV-B时均显示出100%的特异性。与逆转录定量聚合酶链反应（RT-qPCR）相比，RSV-A和RSV-B的诊断准确率分别为90.32%和93.55%。这些数据表明了一种用于RSV筛查的有效策略，显示出在检测多种病毒感染方面的潜在应用前景。
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