Sutherland S S, Evans R J, Bathgate J
Aust Vet J. 1986 Dec;63(12):412-5. doi: 10.1111/j.1751-0813.1986.tb15920.x.
Bovine field serums from the Australian brucellosis eradication program were used to compare 2 enzyme linked immunosorbent assays (ELISA) with the complement fixation test (CFT) and Rose Bengal test (RBT). One ELISA used an anti-bovine IgG horseradish peroxidase conjugate (ELISA 1) and the other a monoclonal anti-bovine Ig alkaline phosphatase conjugate (ELISA 2). When compared with the CFT, the ELISA 2 like the ELISA 1 lacked specificity in B. abortus vaccinated herds but the ELISA 2 was more specific than the ELISA 1 in previously infected herds and equally as specific as the ELISA 1 in nonvaccinated Brucella free herds. In this study the ELISA 2 proved more sensitive than the CFT, RBT and ELISA 1 particularly in herds where B. abortus biotype 2 was present. The value of using the ELISA 2 in conjunction with the CFT in an eradication program is discussed.
来自澳大利亚布鲁氏菌病根除计划的牛血清用于比较两种酶联免疫吸附测定（ELISA）与补体结合试验（CFT）和玫瑰红试验（RBT）。一种ELISA使用抗牛IgG辣根过氧化物酶结合物（ELISA 1），另一种使用单克隆抗牛Ig碱性磷酸酶结合物（ELISA 2）。与CFT相比，ELISA 2与ELISA 1一样，在接种流产布鲁氏菌疫苗的牛群中缺乏特异性，但在先前感染的牛群中，ELISA 2比ELISA 1更具特异性，在未接种疫苗的无布鲁氏菌牛群中与ELISA 1的特异性相同。在本研究中，ELISA 2被证明比CFT、RBT和ELISA 1更敏感，特别是在存在流产布鲁氏菌生物型2的牛群中。讨论了在根除计划中联合使用ELISA 2和CFT的价值。
