Saravi M A, Wright P F, Gregoret R J, Gall D E
Instituto de Bacteriologia, Instituto Nacional de Tecnologia Agropecuarias, Moron Provincia de Buenos Aires, Argentina.
Vet Immunol Immunopathol. 1995 Jul;47(1-2):93-9. doi: 10.1016/0165-2427(94)05353-t.
The FAO/IAEA enzyme-linked immunosorbent assay (ELISA) kit for the diagnosis of bovine brucellosis was compared in Argentina with two screening tests, the Rose Bengal (RB) and the buffered plate antigen (BPA) agglutination tests and with two confirmatory tests, the 2-mercaptoethanol (2-ME) agglutination and the complement fixation (CF) tests. In the testing of Brucella abortus Strain 19 (S19) vaccinated cattle from Brucella-free dairy herds, the diagnostic specificity estimate of the ELISA (99.7%) was shown to be comparable to the RB (99.7%), 2-ME (99.8%) and CF (99.9%) and greater than the BPA (90.6%). In the testing of S19 vaccinated cattle from infected herds, the sensitivity estimates of the BPA (99.5%, 99.6% and 98.6% respectively) relative to CF,2-ME and ELISA positive reactors were comparable and high. The relative sensitivity estimates of the RB (86.3%, 81.4% and 79.1%) in the same comparison were disparate and lower. The ELISA demonstrated the highest relative sensitivity estimates (97.1% and 95.2% respectively) in a three-way comparison between ELISA, CF and 2-ME positive reactors from these herds. Relative to BPA positive reactors from the same infected herds, the sensitivity estimate of the ELISA (57.0%) was comparable to the 2-ME (56.2%) and higher than the CF (51.8%). These results would suggest that the overall diagnostic specificity and sensitivity of the ELISA test is comparable, if not superior, to the tests used to confirm BPA positive reactor status.
粮农组织/国际原子能机构用于诊断牛布鲁氏菌病的酶联免疫吸附测定（ELISA）试剂盒在阿根廷与两种筛查试验（即玫瑰红（RB）试验和缓冲板抗原（BPA）凝集试验）以及两种确证试验（即2-巯基乙醇（2-ME）凝集试验和补体结合（CF）试验）进行了比较。在对来自无布鲁氏菌病奶牛群且接种了流产布鲁氏菌19号菌株（S19）的牛进行检测时，ELISA的诊断特异性估计值（99.7%）与RB（99.7%）、2-ME（99.8%）和CF（99.9%）相当，且高于BPA（90.6%）。在对来自感染牛群且接种了S19的牛进行检测时，BPA相对于CF、2-ME和ELISA阳性反应动物的敏感性估计值（分别为99.5%、99.6%和98.6%）相当且较高。在相同比较中，RB的相对敏感性估计值（分别为86.3%、81.4%和79.1%）则不一致且较低。在对这些牛群中ELISA、CF和2-ME阳性反应动物进行的三方比较中，ELISA显示出最高的相对敏感性估计值（分别为97.1%和95.2%）。相对于来自同一感染牛群的BPA阳性反应动物，ELISA的敏感性估计值（57.0%）与2-ME（56.2%）相当，且高于CF（51.8%）。这些结果表明，ELISA检测的总体诊断特异性和敏感性即便不优于用于确证BPA阳性反应动物状态的检测，至少也是相当的。
