Cargill C, Lee K, Clarke I
Aust Vet J. 1985 Feb;62(2):49-52. doi: 10.1111/j.1751-0813.1985.tb14233.x.
An enzyme-linked immunosorbent assay (ELISA) was developed and was compared with the complement fixation test (CFT) in a bovine brucellosis eradication program. The ELISA detected significantly more reactors than the CFT in both strain 19 vaccinated infected herds (1.79% versus 1.14%) and non-vaccinated infected herds (4.2% versus 3.59%) but not in either vaccinated or non-vaccinated brucella-free herds. The specificity for both tests in brucella-free herds was greater than 0.998. The specificity and sensitivity of the ELISA were compared with those of 3 other tests (the Rose Bengal test; the indirect haemolysis test [IHLT] and the CFT) on serum from 151 animals cultured at slaughter. The calculated specificity of the ELISA in this infected group was lower than for both the CFT and the IHLT (0.58 versus 0.67 versus 0.75). The sensitivity however was much greater (1.0 versus 0.73 versus 0.71). The value of the ELISA when used in an eradication program is discussed.
在一项牛布鲁氏菌病根除计划中，开发了一种酶联免疫吸附测定法（ELISA），并将其与补体结合试验（CFT）进行比较。在19号菌株疫苗接种感染牛群（1.79%对1.14%）和未接种感染牛群（4.2%对3.59%）中，ELISA检测到的反应动物显著多于CFT，但在接种或未接种无布鲁氏菌牛群中均未出现这种情况。在无布鲁氏菌牛群中，两种检测方法的特异性均大于0.998。将ELISA的特异性和敏感性与其他3种检测方法（玫瑰红试验、间接溶血试验[IHLT]和CFT）对151头屠宰时进行培养的动物血清的检测结果进行比较。在这个感染组中，ELISA计算出的特异性低于CFT和IHLT（分别为0.58、0.67和0.75）。然而，其敏感性要高得多（分别为1.0、0.73和0.71）。文中讨论了ELISA在根除计划中的应用价值。
