与血管加压素不同，凝血酶似乎能刺激人血小板中的两种不同的鸟嘌呤核苷酸调节蛋白。
Thrombin, unlike vasopressin, appears to stimulate two distinct guanine nucleotide regulatory proteins in human platelets.
作者信息
Houslay M D, Bojanic D, Gawler D, O'Hagan S, Wilson A
出版信息
Biochem J. 1986 Aug 15;238(1):109-13. doi: 10.1042/bj2380109.
Abstract
The thrombin-stimulated GTPase activity of human platelets was additive with respect to the GTPase stimulation effected by prostaglandin E1, but not with that stimulated by adrenaline, vasopressin and platelet-activating factor (PAF). Treatment of platelet membranes with pertussis toxin partially inhibited the thrombin-stimulated GTPase, but had no effect on the vasopressin-stimulated GTPase activity, whereas cholera toxin treatment had no effect on either of these stimulated GTPase activities. Thrombin, adrenaline and PAF, but not vasopressin, inhibited the adenylate cyclase activity of isolated plasma membranes through the action of Ni only, this being inhibited by pertussis toxin. It is suggested that thrombin exerts effects through both the inhibitory guanine nucleotide regulatory protein Ni and through the putative guanine nucleotide regulatory protein, Np, involved in regulating receptor-stimulated inositol phospholipid metabolism. However, vasopressin appears to exert its effects solely through the putative Np.
摘要
人血小板中凝血酶刺激的GTP酶活性与前列腺素E1刺激的GTP酶活性具有加和性，但与肾上腺素、血管加压素和血小板激活因子（PAF）刺激的GTP酶活性不具有加和性。用百日咳毒素处理血小板膜可部分抑制凝血酶刺激的GTP酶，但对血管加压素刺激的GTP酶活性无影响，而霍乱毒素处理对这两种刺激的GTP酶活性均无影响。凝血酶、肾上腺素和PAF（而非血管加压素）仅通过Ni的作用抑制分离的质膜的腺苷酸环化酶活性，此活性被百日咳毒素抑制。提示凝血酶通过抑制性鸟嘌呤核苷酸调节蛋白Ni以及参与调节受体刺激的肌醇磷脂代谢的假定鸟嘌呤核苷酸调节蛋白Np发挥作用。然而，血管加压素似乎仅通过假定的Np发挥作用。
相似文献
1
Thrombin, unlike vasopressin, appears to stimulate two distinct guanine nucleotide regulatory proteins in human platelets.与血管加压素不同，凝血酶似乎能刺激人血小板中的两种不同的鸟嘌呤核苷酸调节蛋白。
Biochem J. 1986 Aug 15;238(1):109-13. doi: 10.1042/bj2380109.
2
Interactions in platelets between G proteins and the agonists that stimulate phospholipase C and inhibit adenylyl cyclase.血小板中G蛋白与刺激磷脂酶C并抑制腺苷酸环化酶的激动剂之间的相互作用。
J Biol Chem. 1988 Apr 15;263(11):5348-55.
3
Platelet activating factor and U44069 stimulate a GTPase activity in human platelets which is distinct from the guanine nucleotide regulatory proteins, Ns and Ni.血小板活化因子和U44069可刺激人血小板中的一种GTP酶活性，该活性不同于鸟嘌呤核苷酸调节蛋白Ns和Ni。
Biochem J. 1986 Mar 15;234(3):737-40. doi: 10.1042/bj2340737.
4
Evidence for two GTPases activated by thrombin in membranes of human platelets.人血小板膜中两种由凝血酶激活的GTP酶的证据。
Biochem J. 1986 Aug 1;237(3):669-74. doi: 10.1042/bj2370669.
5
Insulin stimulates a novel GTPase activity in human platelets.
FEBS Lett. 1987 May 25;216(1):94-8. doi: 10.1016/0014-5793(87)80763-9.
6
Ca2+ ionophore A23187 and thrombin inhibit the pertussis-toxin-induced ADP-ribosylation of the alpha-subunit of the inhibitory guanine-nucleotide-binding protein and other proteins in human platelets.钙离子载体A23187和凝血酶可抑制百日咳毒素诱导的人血小板中抑制性鸟嘌呤核苷酸结合蛋白α亚基及其他蛋白质的ADP核糖基化。
Eur J Biochem. 1991 Nov 15;202(1):145-50. doi: 10.1111/j.1432-1033.1991.tb16355.x.
7
Regulation of the phosphoinositide hydrolysis pathway in thrombin-stimulated platelets by a pertussis toxin-sensitive guanine nucleotide-binding protein. Evaluation of its contribution to platelet activation and comparisons with the adenylate cyclase inhibitory protein, Gi.百日咳毒素敏感的鸟嘌呤核苷酸结合蛋白对凝血酶刺激的血小板中磷酸肌醇水解途径的调节。评估其对血小板活化的作用并与腺苷酸环化酶抑制蛋白Gi进行比较。
J Biol Chem. 1986 Dec 25;261(36):16838-47.
8
Activation of the cloned platelet thrombin receptor decreases the pertussis-toxin-dependent ADP-ribosylation of the membrane and soluble inhibitory guanine-nucleotide-binding-alpha proteins. Inhibition by the prostacyclin analog, iloprost.克隆化血小板凝血酶受体的激活降低了膜和可溶性抑制性鸟嘌呤核苷酸结合α蛋白的百日咳毒素依赖性ADP核糖基化。前列环素类似物伊洛前列素的抑制作用。
Eur J Biochem. 1993 Aug 15;216(1):81-8. doi: 10.1111/j.1432-1033.1993.tb18119.x.
9
Islet-activating protein impairs alpha 2-adrenoceptor-mediated inhibitory regulation of human platelet adenylate cyclase.胰岛激活蛋白损害α2-肾上腺素能受体介导的人血小板腺苷酸环化酶的抑制性调节。
Naunyn Schmiedebergs Arch Pharmacol. 1983 Nov;324(3):196-200. doi: 10.1007/BF00503894.
10
Ni-mediated inhibition of human platelet adenylate cyclase by thrombin.镍介导凝血酶对人血小板腺苷酸环化酶的抑制作用。
Eur J Biochem. 1984 Dec 3;145(2):333-8. doi: 10.1111/j.1432-1033.1984.tb08558.x.
引用本文的文献
1
Inhibition of human platelet adenylate cyclase activity by adrenaline, thrombin and collagen: analysis and reinterpretation of experimental data.肾上腺素、凝血酶和胶原蛋白对人血小板腺苷酸环化酶活性的抑制作用：实验数据的分析与重新解读
Biochem J. 1999 May 15;340 ( Pt 1)(Pt 1):245-53.
2
Signalling functions and biochemical properties of pertussis toxin-resistant G-proteins.百日咳毒素抗性G蛋白的信号传导功能及生化特性
Biochem J. 1997 Feb 1;321 ( Pt 3)(Pt 3):561-71. doi: 10.1042/bj3210561.
3
The role of Gi and the membrane-fluidizing agent benzyl alcohol in modulating the hysteretic activation of human platelet adenylate cyclase by guanylyl 5'-imidodiphosphate.Gi和膜流化剂苯甲醇在通过5'-亚氨基二磷酸鸟苷调节人血小板腺苷酸环化酶的滞后激活中的作用。
Biochem J. 1993 May 1;291 ( Pt 3)(Pt 3):945-9. doi: 10.1042/bj2910945.
4
Pharmacological differentiation by pertussis toxin of the in vivo acute responses to fMLP and PAF in guinea-pig lungs.百日咳毒素对豚鼠肺脏中fMLP和PAF体内急性反应的药理学区分
Br J Pharmacol. 1993 Feb;108(2):412-7. doi: 10.1111/j.1476-5381.1993.tb12818.x.
5
Thromboxane-insensitive dog platelets have impaired activation of phospholipase C due to receptor-linked G protein dysfunction.对血栓烷不敏感的犬血小板由于受体连接的G蛋白功能障碍，磷脂酶C的激活受损。
J Clin Invest. 1993 Nov;92(5):2469-79. doi: 10.1172/JCI116855.
6
Platelet adenylate cyclase and phospholipase C are affected differentially by ADP-ribosylation. Effects on thrombin-mediated responses.血小板腺苷酸环化酶和磷脂酶C受ADP-核糖基化的影响不同。对凝血酶介导反应的影响。
Biochem J. 1988 May 15;252(1):297-300. doi: 10.1042/bj2520297.
7
Inositol 1,4,5-triphosphate-induced granule secretion in platelets. Evidence that the activation of phospholipase C mediated by platelet thromboxane receptors involves a guanine nucleotide binding protein-dependent mechanism distinct from that of thrombin.肌醇1,4,5-三磷酸诱导血小板颗粒分泌。有证据表明，由血小板血栓素受体介导的磷脂酶C激活涉及一种与凝血酶不同的鸟嘌呤核苷酸结合蛋白依赖性机制。
J Clin Invest. 1987 Apr;79(4):1269-75. doi: 10.1172/JCI112947.
8
Heparin and heparinoids impair adrenaline and platelet-activating factor but not thrombin-induced inhibition of adenylate cyclase and stimulation of GTP hydrolysis in human platelet membranes.肝素及类肝素可削弱肾上腺素和血小板活化因子的作用，但不影响凝血酶对人血小板膜中腺苷酸环化酶的抑制作用及对鸟苷三磷酸水解的刺激作用。
Naunyn Schmiedebergs Arch Pharmacol. 1988 Mar;337(3):354-8. doi: 10.1007/BF00168850.
9
Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins.
Biochem J. 1988 Oct 1;255(1):1-13. doi: 10.1042/bj2550001.
10
Antibodies which recognize the C-terminus of the inhibitory guanine-nucleotide-binding protein (Gi) demonstrate that opioid peptides and foetal-calf serum stimulate the high-affinity GTPase activity of two separate pertussis-toxin substrates.识别抑制性鸟嘌呤核苷酸结合蛋白（Gi）C端的抗体表明，阿片肽和胎牛血清可刺激两种不同百日咳毒素底物的高亲和力GTP酶活性。
Biochem J. 1988 Feb 1;249(3):653-9. doi: 10.1042/bj2490653.
本文引用的文献
1
Thrombin-induced hydrolysis of phosphatidylinositol in human platelets.凝血酶诱导的人血小板中磷脂酰肌醇的水解。
J Biol Chem. 1980 Mar 10;255(5):1790-2.
2
Ni-mediated inhibition of human platelet adenylate cyclase by thrombin.镍介导凝血酶对人血小板腺苷酸环化酶的抑制作用。
Eur J Biochem. 1984 Dec 3;145(2):333-8. doi: 10.1111/j.1432-1033.1984.tb08558.x.
3
Platelet-activating factor stimulates phosphatidylinositol turnover in human platelets.血小板激活因子刺激人血小板中的磷脂酰肌醇周转。
Biochem J. 1983 May 15;212(2):433-7. doi: 10.1042/bj2120433.
4
The phorbol ester, TPA inhibits glucagon-stimulated adenylate cyclase activity.佛波酯TPA可抑制胰高血糖素刺激的腺苷酸环化酶活性。
FEBS Lett. 1984 May 7;170(1):38-42. doi: 10.1016/0014-5793(84)81364-2.
5
G proteins and dual control of adenylate cyclase.G蛋白与腺苷酸环化酶的双重调控
Cell. 1984 Mar;36(3):577-9. doi: 10.1016/0092-8674(84)90336-2.
6
Vasopressin inhibits the adenylate cyclase activity of human platelet particulate fraction through V1-receptors.血管加压素通过V1受体抑制人血小板微粒体部分的腺苷酸环化酶活性。
FEBS Lett. 1983 Dec 12;164(2):340-4. doi: 10.1016/0014-5793(83)80313-5.
7
Forskolin and ethanol both perturb the structure of liver plasma membranes and activate adenylate cyclase activity.福斯高林和乙醇都会扰乱肝细胞膜的结构并激活腺苷酸环化酶活性。
Biochem Pharmacol. 1983 May 15;32(10):1601-8. doi: 10.1016/0006-2952(83)90334-9.
8
Stimulation and inhibition of adenylyl cyclases mediated by distinct regulatory proteins.由不同调节蛋白介导的腺苷酸环化酶的激活与抑制
Nature. 1983 Apr 21;302(5910):706-9. doi: 10.1038/302706a0.
9
Thrombin-induced phosphodiesteratic cleavage of phosphatidylinositol bisphosphate in human platelets.凝血酶诱导人血小板中磷脂酰肌醇二磷酸的磷酸二酯酶裂解
J Biol Chem. 1983 Feb 25;258(4):2076-8.
10
Rapid decrease of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.凝血酶刺激的血小板中磷脂酰肌醇4,5-二磷酸的快速减少。
J Biol Chem. 1982 Nov 10;257(21):12705-8.
Zotero 插件