Janelia Research Campus , Howard Hughes Medical Institute , 19700 Helix Drive , Ashburn , Virginia 20147 , United States.
ACS Chem Biol. 2019 Jun 21;14(6):1077-1090. doi: 10.1021/acschembio.9b00197. Epub 2019 May 13.
Development of single-molecule localization microscopy (SMLM) has sparked a revolution in biological imaging, allowing "super-resolution" fluorescence microscopy below the diffraction limit of light. The past decade has seen an explosion in not only optical hardware for SMLM but also the development or repurposing of fluorescent proteins and small-molecule fluorescent probes for this technique. In this review, written by chemists for chemists, we detail the history of single-molecule localization microscopy and collate the collection of probes with demonstrated utility in SMLM. We hope it will serve as a primer for probe choice in localization microscopy as well as an inspiration for the development of new fluorophores that enable imaging of biological samples with exquisite detail.
单分子定位显微镜（SMLM）的发展引发了生物学成像领域的革命，使得光的衍射极限以下的“超分辨率”荧光显微镜成为可能。在过去的十年中，不仅 SMLM 的光学硬件得到了爆炸式的发展，而且用于该技术的荧光蛋白和小分子荧光探针也得到了开发或重新利用。在这篇由化学家撰写的评论中，我们详细介绍了单分子定位显微镜的历史，并整理了具有 SMLM 实用价值的探针集合。我们希望它能成为定位显微镜中探针选择的入门指南，也能为开发新的荧光染料提供灵感，使具有精美细节的生物样品成像成为可能。
