Brown M S, Peters S P, Adkinson N F, Proud D, Kagey-Sobotka A, Norman P S, Lichtenstein L M, Naclerio R M
Arch Otolaryngol Head Neck Surg. 1987 Feb;113(2):179-83. doi: 10.1001/archotol.1987.01860020071015.
In order to assess the role of arachidonic acid metabolites in the early reaction to antigen, we challenged six allergic individuals with and without premedication with aspirin and recorded their clinical response, as indicated by number of sneezes, and measured the levels of inflammatory mediators. The early reaction to antigen was associated with increases in the levels of histamine, N-alpha-tosyl-L-arginine methyl esterase (TAME-esterase) activity, prostaglandin (PG) D2, leukotriene C4, PGE, and thromboxane. Aspirin significantly inhibited the increases in the cyclooxygenase metabolites PGE, PGD2, PGF2 alpha, 6-keto-PGF1 alpha, and thromboxane but did not affect the amount of sneezing or the levels of histamine, TAME-esterase activity, or leukotrienes. The pattern of the metabolites and their response to pretreatment with aspirin parallel the response of purified human lung mast cells, supporting the notion that the early phase of allergic rhinitis is a mast cell-dominated event.
为了评估花生四烯酸代谢产物在抗原早期反应中的作用，我们对6名过敏个体进行了试验，一组预先服用阿司匹林，另一组未服用，记录他们的临床反应（以打喷嚏次数表示），并测量炎症介质的水平。对抗原的早期反应与组胺水平升高、N-α-对甲苯磺酰-L-精氨酸甲酯酶（TAME-酯酶）活性升高、前列腺素（PG）D2、白三烯C4、PGE和血栓素有关。阿司匹林显著抑制了环氧化酶代谢产物PGE、PGD2、PGF2α、6-酮-PGF1α和血栓素的升高，但不影响打喷嚏次数或组胺水平、TAME-酯酶活性或白三烯水平。代谢产物的模式及其对阿司匹林预处理的反应与纯化的人肺肥大细胞的反应相似，这支持了变应性鼻炎早期是肥大细胞主导事件的观点。
