He Wen-Hua
Department of Hematology, Suining Central Hospital of Sichuan, Suining 629000, Sichuan Province, China,E-mail：
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Apr;27(2):415-420. doi: 10.19746/j.cnki.issn.1009-2137.2019.02.017.
To investigate the expression change of ROCK1 gene in patients with acute lymphoblastic leukemia (ALL) and its prognostic significance.
Sixty patients with ALL were selected in our hospital from April 2017 to April 2018, and 60 healthy persons subjected to physical examination were selected as control. The venous blood was taken from the subjects, and then the mononuclear cells were separated. The ROCK1 gene expression level in the samples was detected by RT-PCR, and the expression level of ROCK1 protein was detected by Western blot. The correlation between ROCK1 gene expression and clinical characteristics of ALL patients was analyzed by using statistical methots.
The RT-PCR showed that the relative expression level of ROCK1 gene in ALL patients was 1.37 (1.28-1.46), which was significantly higher than that in the control group (P<0.05). Western blot showed that the protein expression level of ROCK1 in ALL patients was higher than that in the control group (P<0.05). The expression level of ROCK1 gene correlated with age, WBC count, lactate dehydrogenase (LDH) level, peripheral blood immature cell count, and risk stratification of ALL patients (P<0.05). The expression level of ROCK1 gene did not correlate with sex, hemoglobin (Hb) level, platelet count and immunophenotype in ALL patients (P>0.05). The standard risk ratio of B-ALL and T-ALL patients with low ROCK1 expression was significantly higher than that in patients with high ROCK1 expression (P<0.05). The high risk ratio of B-ALL and T-ALL patients with low ROCK1 expression was significantly lower than those with high ROCK1 expression (P<0.05). The ratio of CR in the group with low ROCK1 expression patients was significantly higher than that in patients with high ROCK1 expression (P<0.05). The Relapse rate of the group with low ROCK1 expression was significantly lower than that of the group with high ROCK1 expression (P<0.05). Kaplan-Meier survival analysis showed that OS and DFS in ALL patients with low ROCK1 expression were superior to those in ALL patients with high ROCK1 expression (P<0.05). Multiple factor Cox regression analysis showed that age and ROCK1 gene were independent influencing factors for OS (P<0.05); leukocyte count and ROCK1 gene were independent influencing factors for DFS (P<0.05).
The expression level of ROCK1 gene in ALL patients is high, which may stimulate the genesis of ALL, and the down-regulation of ROCK1 gene expression may help improve the therapeutic effect for ALL patients.
探讨Rho相关卷曲螺旋蛋白激酶1（ROCK1）基因在急性淋巴细胞白血病（ALL）患者中的表达变化及其预后意义。
选取2017年4月至2018年4月在我院就诊的60例ALL患者，并选取60例健康体检者作为对照。采集受试者静脉血，分离单核细胞。采用逆转录聚合酶链反应（RT-PCR）检测样本中ROCK1基因表达水平，采用蛋白质免疫印迹法（Western blot）检测ROCK1蛋白表达水平。运用统计学方法分析ROCK1基因表达与ALL患者临床特征的相关性。
RT-PCR结果显示，ALL患者ROCK1基因相对表达水平为1.37（1.28～1.46），明显高于对照组（P<0.05）。Western blot结果显示，ALL患者ROCK1蛋白表达水平高于对照组（P<0.05）。ROCK1基因表达水平与ALL患者的年龄、白细胞计数、乳酸脱氢酶（LDH）水平、外周血幼稚细胞计数及危险分层相关（P<0.05）。ROCK1基因表达水平与ALL患者的性别、血红蛋白（Hb）水平、血小板计数及免疫表型无关（P>0.05）。ROCK1表达水平低的B-ALL和T-ALL患者的标准危险比显著高于ROCK1表达水平高的患者（P<0.05）。ROCK1表达水平低的B-ALL和T-ALL患者的高危比显著低于ROCK1表达水平高的患者（P<0.05）。ROCK1表达水平低的患者组完全缓解（CR）率显著高于ROCK1表达水平高的患者（P<0.05）。ROCK1表达水平低的患者组复发率显著低于ROCK1表达水平高的患者组（P<0.05）。Kaplan-Meier生存分析显示，ROCK1表达水平低的ALL患者总生存期（OS）和无病生存期（DFS）均优于ROCK1表达水平高的ALL患者（P<0.05）。多因素Cox回归分析显示，年龄和ROCK1基因是OS的独立影响因素（P<0.05）；白细胞计数和ROCK1基因是DFS的独立影响因素（P<0.05）。
ALL患者ROCK1基因表达水平较高，可能促进ALL的发生，下调ROCK1基因表达可能有助于提高ALL患者的治疗效果。
