Zhang Li-Cui, Zhong Chen, Ma Ya-Jing, Liu Min, Nong Wei-Xia
Department of Clinical Laboratorial Examination, The First Affiliated Hospital of Medical College of Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China.
Third Department of General Surgery, The First Affiliated Hospital of Medical College of Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Feb;28(1):119-124. doi: 10.19746/j.cnki.issn.1009-2137.2020.01.020.
To analyze the expression level of miR-429 in patients with acute lymphoblastic leukemia(ALL) and its clinical prognostic value.
One hundred and Twenty-six patients with ALL treated in our hospital from April 2016 to February 2018 were selected, and 100 healthy persons in the same period were selected as control group. Bone marrow mononuclear cells were collected. The expression level of miR-429 in bone marrow mononuclear cells was detected by RT-PCR, and the correlation of miR-429 expression with clinical characteristics and therapeutic efficacy was analyzed. Kaplan-Meier method and multi-factorial Cox regression model were used to analyze the correlation between the level of microRNA-429 and the prognosis of ALL patients.
The relative level of miR-429 in ALL patients was 2.47±0.07, which was signifi-cantly higher than that in control group (P<0.05). The level of miR-429 significantly correlated with the leucocyte level（r=0.994）, LDH（r=0.992）, the ratio of bone marrow primordial cells（r=0.995） and risk grade of ALL patients（r=0.991). The level of miR-429 not correlate with the age, sex, Hb level, Plt level and immunophenotype of ALL patients (P>0.05). The level of miR-429 was not significantly different between CR patients and control group (P>0.05); the level of miR-429 in PR patients was higher than that in control group and CR patients (P<0.05). The level of miR-429 in NR patients was higher than that in other groups (P<0.05). Kaplan-Meier survival analysis showed that the overall survival rate of ALL patients with low expression of miR-429 was better than that of ALL patients with high expression of miR-429 (P<0.05). Univariate Cox regression analysis showed that leukocyte level, ratio of bone marrow primordial cells, Hb and LDH level, risk grading and miR-429 were the factors influencing overall survival rate in ALL patients (P<0.05). Multivariate Cox regression analysis showed that leukocyte level, ratio of bone marrow primordial cells, risk grading, and miR-429 were the factors influencing overall survival rate (P<0.05).
The expression of miR-429 is high in ALL patients, which closely relates to the curative effect and pro-gnosis of ALL patients, and can be used as a reference index for evaluation of clinical prognosis of ALL patients.
分析急性淋巴细胞白血病（ALL）患者中miR-429的表达水平及其临床预后价值。
选取2016年4月至2018年2月在我院治疗的126例ALL患者，并选取同期100例健康人作为对照组。采集骨髓单个核细胞。采用RT-PCR检测骨髓单个核细胞中miR-429的表达水平，并分析miR-429表达与临床特征及治疗效果的相关性。采用Kaplan-Meier法和多因素Cox回归模型分析微小RNA-429水平与ALL患者预后的相关性。
ALL患者中miR-429的相对水平为2.47±0.07，显著高于对照组（P<0.05）。miR-429水平与ALL患者的白细胞水平（r=0.994）、乳酸脱氢酶（LDH）（r=0.992）、骨髓原始细胞比例（r=0.995）及危险度分级（r=0.991）显著相关。miR-429水平与ALL患者的年龄、性别、血红蛋白（Hb）水平、血小板（Plt）水平及免疫表型无关（P>0.05）。完全缓解（CR）患者与对照组之间miR-429水平无显著差异（P>0.05）；部分缓解（PR）患者的miR-429水平高于对照组和CR患者（P<0.05）。未缓解（NR）患者的miR-429水平高于其他组（P<0.05）。Kaplan-Meier生存分析显示，miR-429低表达的ALL患者总生存率优于miR-429高表达的ALL患者（P<0.05）。单因素Cox回归分析显示，白细胞水平、骨髓原始细胞比例、Hb和LDH水平、危险度分级及miR-429是影响ALL患者总生存率的因素（P<0.05）。多因素Cox回归分析显示，白细胞水平、骨髓原始细胞比例、危险度分级及miR-429是影响总生存率的因素（P<0.05）。
ALL患者中miR-429表达较高，与ALL患者的疗效和预后密切相关，可作为评估ALL患者临床预后的参考指标。
