Gemke R J, Kanhai H H, Overbeeke M A, Maas C J, Bennebroek Gravenhorst J, Bernini L F, Engelfriet C P, van't Veer M B
Br J Haematol. 1986 Dec;64(4):689-97. doi: 10.1111/j.1365-2141.1986.tb02230.x.
Determination of fetal red blood cell antigens in early pregnancy can be important in cases with a history of severe haemolytic disease of the newborn. From chorionic villus biopsies (CVB) between the 8th and 12th week of gestation a small number of fetal red blood cells was obtained, inevitably highly contaminated with maternal blood cells. Two techniques were used to demonstrate the minor (fetal) cell population with a blood group antigen differing from the major (maternal) cell population: a solid-phase microfluorescence technique (introduced in this paper) which was compared with the mixed agglutination technique. In series of artificial mixtures of erythrocytes it was shown that with the microfluorescence technique the ABO and Rhesus phenotypes of minor cell populations could be determined at a ratio of 1 in 4000 erythrocytes of the major population, making this technique 4 times as sensitive as the mixed agglutination technique. We further investigated the reliability of the microfluorescence technique to demonstrate antagonistic fetal blood groups in the first trimester of pregnancy. Of 18 women undergoing CVB prior to therapeutic abortion, blood group antagonism (ABO and Rhesus systems) was demonstrate in all 11 cases in which it was present. Therefore, it seems that CVB can be reliably used for the prenatal diagnosis of (recurrent) blood group antagonism.
对于有新生儿严重溶血病病史的病例，在妊娠早期测定胎儿红细胞抗原可能具有重要意义。在妊娠第8至12周期间进行绒毛膜绒毛活检（CVB），可获得少量胎儿红细胞，但不可避免地会被母体血细胞高度污染。使用了两种技术来显示具有与主要（母体）细胞群不同血型抗原的次要（胎儿）细胞群：一种固相微荧光技术（本文介绍），并与混合凝集技术进行了比较。在一系列红细胞人工混合物中表明，使用微荧光技术，次要细胞群的ABO和恒河猴血型表型可以在主要群体的4000个红细胞中有1个的比例下确定，这使得该技术的灵敏度是混合凝集技术的4倍。我们进一步研究了微荧光技术在妊娠早期显示胎儿血型拮抗的可靠性。在18例治疗性流产前行CVB的妇女中，在所有存在血型拮抗（ABO和恒河猴系统）的11例病例中均得到了证实。因此，似乎CVB可可靠地用于（复发性）血型拮抗的产前诊断。
