Kickler T S, Blakemore K, Shirey R S, Nicol S, Callan N, Ness P M, Escallon C, Dover G
Department of Laboratory Medicine, Johns Hopkins University School of Medicine, Baltimore, MD.
Am J Obstet Gynecol. 1992 May;166(5):1407-11. doi: 10.1016/0002-9378(92)91612-e.
The prenatal Rh typing of red blood cells obtained by chorionic villus sampling was performed with an immune rosette technique.
Ten Rh-negative pregnant women undergoing chorionic villus sampling at 9 to 11 weeks' gestation were studied at a large referral hospital.
Accurate Rh phenotyping may be done on red blood cells obtained from chorionic villi weighing 2 to 8 mg. The preparations were shown to be greater than 90% fetal in origin as demonstrated by radial immunodiffusion quantitation of fetal hemoglobin-containing cells. Of the 10 fetuses typed in the first trimester nine of the pregnancies were carried to term. In all cases typing of red blood cells confirmed the antenatal fetal red cell typing.
This study shows that antenatal Rh phenotyping may be performed as early as 9 to 11 weeks' gestation. This technique may be used in the management of pregnancies complicated by Rh incompatibility.
采用免疫玫瑰花结技术对经绒毛取样获得的红细胞进行产前Rh血型鉴定。
在一家大型转诊医院对10名妊娠9至11周接受绒毛取样的Rh阴性孕妇进行了研究。
对重量为2至8毫克的绒毛膜绒毛所获得的红细胞可进行准确的Rh表型分析。通过对含胎儿血红蛋白细胞的放射免疫扩散定量分析表明，所制备的样本90%以上来源于胎儿。在孕早期进行血型鉴定的10例胎儿中，有9例妊娠至足月。在所有病例中，红细胞分型均证实了产前胎儿红细胞分型。
本研究表明，早在妊娠9至11周即可进行产前Rh表型分析。该技术可用于Rh血型不相容性并发症妊娠的管理。
