Sinclair D, Dagg J H, Dewar A E, Mowat A M, Parrott D M, Stockdill G, Stott D I
Br J Haematol. 1986 Dec;64(4):725-35. doi: 10.1111/j.1365-2141.1986.tb02234.x.
Immuno-isoelectric focusing (IIEF) showed a 61% incidence of serum paraproteinaemia in 56 patients with chronic lymphocytic leukaemia (CLL). A strong correlation between the serum paraprotein heavy chain isotypes and those of the cytoplasmic heavy chain immunoglobulins was observed with no discrepancy noted in light chain expression. Density gradient ultracentrifugation analysis of selected sera containing monoclonal IgM showed that the IgM paraproteins were mostly 19S, secretory IgM but one patient was found to have both 19S and 8S monoclonal IgM. When the cellular origin of the IgM and IgD paraproteins found in one patient was investigated, both paraproteins were found to share the same idiotype and originate from the neoplastic clone. These findings confirm the view that there is an incomplete maturation block in chronic lymphocytic leukaemia and that in vivo secretion of paraproteins by the neoplastic cells is a relatively common occurrence.
免疫等电聚焦（IIEF）显示，56例慢性淋巴细胞白血病（CLL）患者中血清副蛋白血症的发生率为61%。观察到血清副蛋白重链同种型与细胞质重链免疫球蛋白的重链同种型之间存在强相关性，轻链表达未发现差异。对选定的含有单克隆IgM的血清进行密度梯度超速离心分析表明，IgM副蛋白大多为19S分泌型IgM，但发现1例患者同时有19S和8S单克隆IgM。当对1例患者中发现的IgM和IgD副蛋白的细胞来源进行研究时，发现两种副蛋白具有相同的独特型，且均起源于肿瘤克隆。这些发现证实了慢性淋巴细胞白血病存在不完全成熟阻滞的观点，并且肿瘤细胞在体内分泌副蛋白是一种相对常见的现象。
