Basic and Clinical Immunology, Department of Pathology, Faculty of Medicine, University of Porto, Porto, Portugal.
Serviço de Imunoalergologia, Centro Hospitalar de São João E.P.E., Porto, Portugal.
PLoS One. 2019 Apr 18;14(4):e0215475. doi: 10.1371/journal.pone.0215475. eCollection 2019.
Adipose tissue-derived adipokines are pro-inflammatory cytokines involved in metabolic-related diseases and can be influenced by diet and exercise. We aimed to compare the effect of a Mediterranean (MdM) compared with Fast Food (FFM) meal on the exercise induced adipokines changes.
In a double blinded cross over trial, 46 participants were randomly assigned to one of two standardized iso-energy pre-exercise meals: FFM or MdM-type. Three hours after each meal, participants completed a treadmill exercise test (EC). Serum adiponectin, resistin, PAI-1, lipocalin-2/NGAL and adipsin were determined by Luminex magnetic bead immunoassay. Wilcoxon signed rank test compared changes before/after meal and before/after EC and a linear mixed model evaluated the effect of meals on the adipokine response to exercise, adjusted for confounders.
Thirty-nine participants (mean age of 25, with a standard deviation of 5 years) completed the trial (56% females). For both interventions, a significant reduction of adipsin after each meal and a significant increase of lipocalin, PAI-1, adipsin and resistin, after exercise was observed. When exercise was preceded by a MdM meal a higher increase in adipsin levels was seen.
Acute exercise induced an increase of circulatory levels of adipsin, resistin, lipocalin and PAI-1, but not adiponectin. A pre-exercise Mediterranean meal potentiated the increase of adipsin after the exercise test, which possibly relates to the immune regulatory role of adipsin. These changes suggest a cross-talk between the immune and metabolic immediate response to exercise and its modulation by the pre-exercise diet composition.
脂肪组织来源的脂肪因子是与代谢相关疾病有关的促炎细胞因子，可受饮食和运动影响。我们旨在比较地中海饮食（MdM）与快餐（FFM）餐对运动诱导的脂肪因子变化的影响。
在一项双盲交叉试验中，46 名参与者被随机分配到两种标准化等能量的预运动餐中：FFM 或 MdM 型。每餐 3 小时后，参与者完成跑步机运动测试（EC）。通过 Luminex 磁珠免疫测定法测定血清脂联素、抵抗素、PAI-1、脂联素和脂联素。Wilcoxon 符号秩检验比较餐前/餐后和运动前/后餐的变化，线性混合模型评估餐对运动后脂肪因子反应的影响，调整混杂因素。
39 名参与者（平均年龄 25 岁，标准差为 5 岁）完成了试验（56%为女性）。对于两种干预措施，在每次进餐后，脂联素水平显著降低，运动后脂联素、抵抗素、脂联素和 PAI-1 水平显著升高。当运动前吃 MdM 餐时，脂联素水平升高更为明显。
急性运动引起循环中脂联素、抵抗素、脂联素和 PAI-1 水平升高，但不引起脂联素升高。运动前的地中海饮食增强了运动后脂联素水平的升高，这可能与脂联素的免疫调节作用有关。这些变化提示免疫和代谢对运动的即时反应之间存在交叉对话，以及运动前饮食成分对其的调节作用。
