Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo, Japan.
Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo, Japan.
Curr Opin Cell Biol. 2019 Oct;60:19-26. doi: 10.1016/j.ceb.2019.03.005. Epub 2019 Apr 15.
How linear DNA molecules are packaged into compact cylindrical chromosomes in preparation for cell division has remained one of the central outstanding questions in cell biology. Condensin is a highly conserved protein complex that universally determines large-scale DNA geometry during mitotic chromosome assembly. A wide range of recently developed approaches, including super resolution microscopy, single molecule imaging, Hi-C analyses and computational modeling, have profoundly changed how we view mitotic chromosomes. This review highlights recent discoveries on chromosome architecture and condensin function.
线性 DNA 分子如何包装成紧凑的圆柱形染色体，以便为细胞分裂做准备，这一直是细胞生物学中的核心悬而未决的问题之一。凝缩蛋白是一种高度保守的蛋白质复合物，在有丝分裂染色体组装过程中普遍决定着大规模的 DNA 几何形状。包括超分辨率显微镜、单分子成像、Hi-C 分析和计算建模在内的一系列新方法的出现，极大地改变了我们对有丝分裂染色体的看法。本文综述了近年来在染色体结构和凝缩蛋白功能方面的发现。
