State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150000, China.
Int J Mol Sci. 2019 Apr 17;20(8):1895. doi: 10.3390/ijms20081895.
Variegated plants are valuable materials for investigating leaf color regulated mechanisms. To unveil the role of posttranslational modification in the variegated phenotype, we conducted global quantitative phosphoproteomic analysis on different leaf color sectors of and the corresponding of using Ti-IMAC phosphopeptide enrichment. A total of 3778 phosphorylated sites assigned to 1646 phosphoproteins were identified, and 3221 in 1434 proteins were quantified. Differential phosphoproteins (above 1.5 or below 1/1.5) in various leaf color sectors were selected for functional enrichment analyses. Gene ontology (GO) enrichment revealed that processes of photosynthesis, regulation of the generation of precursor metabolites, response to stress, homeostasis, amino acid metabolism, transport-related processes, and most of the energy metabolisms might contribute to leaf color. KEGG pathway enrichment analysis was performed based on differential phosphoproteins (DPs) in different organelles. The result showed that most enriched pathways were located in the chloroplasts and cytosol. The phosphorylation levels of glycometabolism enzymes might greatly affect leaf variegation. Measurements of fluorescence parameters and enzyme activities confirmed that protein phosphorylation could affect plant physiology by regulating enzyme activity. These results provide new clues for further study the formation mechanisms of naturally variegated phenotype.
斑驳植物是研究叶片颜色调控机制的有价值的材料。为了揭示翻译后修饰在斑驳表型中的作用，我们使用 Ti-IMAC 磷酸肽富集法，对 和 的不同叶片颜色区域进行了全局定量磷酸化蛋白质组分析。共鉴定到 3778 个磷酸化位点，分配到 1646 个磷酸化蛋白中，其中 3221 个在 1434 个蛋白中定量。选择不同叶片颜色区域的差异磷酸化蛋白（大于 1.5 或小于 1/1.5）进行功能富集分析。GO 富集分析表明，光合作用、前体代谢物生成的调控、应激反应、内稳态、氨基酸代谢、运输相关过程以及大多数能量代谢过程可能对叶片颜色有贡献。基于不同细胞器中的差异磷酸化蛋白（DPs）进行了 KEGG 途径富集分析。结果表明，大多数富集途径位于叶绿体和细胞质中。糖代谢酶的磷酸化水平可能极大地影响叶片斑驳。荧光参数和酶活性的测量证实，蛋白质磷酸化可以通过调节酶活性来影响植物的生理。这些结果为进一步研究自然斑驳表型的形成机制提供了新线索。
