Zhao Qi, Chen Wenxin, Bian Jiayi, Xie Hao, Li Ying, Xu Chenxi, Ma Jun, Guo Siyi, Chen Jiaying, Cai Xiaofeng, Wang Xiaoli, Wang Quanhua, She Yimin, Chen Sixue, Zhou Zhiqiang, Dai Shaojun
Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, China.
Key Laboratory of Forest Plant Ecology, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin, China.
Front Plant Sci. 2018 Jun 26;9:800. doi: 10.3389/fpls.2018.00800. eCollection 2018.
Elevated temperatures limit plant growth and reproduction and pose a growing threat to agriculture. Plant heat stress response is highly conserved and fine-tuned in multiple pathways. Spinach ( L.) is a cold tolerant but heat sensitive green leafy vegetable. In this study, heat adaptation mechanisms in a spinach sibling inbred heat-tolerant line Sp75 were investigated using physiological, proteomic, and phosphoproteomic approaches. The abundance patterns of 911 heat stress-responsive proteins, and phosphorylation level changes of 45 phosphoproteins indicated heat-induced calcium-mediated signaling, ROS homeostasis, endomembrane trafficking, and cross-membrane transport pathways, as well as more than 15 transcription regulation factors. Although photosynthesis was inhibited, diverse primary and secondary metabolic pathways were employed for defense against heat stress, such as glycolysis, pentose phosphate pathway, amino acid metabolism, fatty acid metabolism, nucleotide metabolism, vitamin metabolism, and isoprenoid biosynthesis. These data constitute a heat stress-responsive metabolic atlas in spinach, which will springboard further investigations into the sophisticated molecular mechanisms of plant heat adaptation and inform spinach molecular breeding initiatives.
高温限制植物生长和繁殖，对农业构成日益严重的威胁。植物热应激反应在多个途径中高度保守且经过精细调节。菠菜（L.）是一种耐寒但对热敏感的绿叶蔬菜。在本研究中，使用生理、蛋白质组学和磷酸蛋白质组学方法研究了菠菜同胞自交耐热系Sp75中的热适应机制。911种热应激反应蛋白的丰度模式以及45种磷酸化蛋白的磷酸化水平变化表明了热诱导的钙介导信号传导、活性氧稳态、内膜运输和跨膜运输途径，以及超过15种转录调节因子。尽管光合作用受到抑制，但多种初级和次级代谢途径被用于抵御热应激，如糖酵解、磷酸戊糖途径、氨基酸代谢、脂肪酸代谢、核苷酸代谢、维生素代谢和类异戊二烯生物合成。这些数据构成了菠菜中的热应激反应代谢图谱，将为进一步研究植物热适应的复杂分子机制提供跳板，并为菠菜分子育种计划提供信息。
