Tantray Javeed Ahmad, Reddy K Pratap, Jamil Kaiser, Yerra Shiva Kumar
Department of Zoology, Osmania University Hyderabad, Telangana, India; Dept. of Genetics, Bhagwan Mahavir Medical Research Centre, 10-1-1, Mahavir Marg, Hyderabad-500004, Telangana, India.
Department of Zoology, Osmania University Hyderabad, Telangana, India.
Indian Heart J. 2019 Jan-Feb;71(1):60-64. doi: 10.1016/j.ihj.2018.11.011. Epub 2018 Dec 1.
The cytochrome P-450 2J2 (CYP2J2) is known to be one of the major enzymes of epoxygenase pathway of arachidonic acid in extrahepatic tissues, which produces series of regioisomeric cis-epoxyeicosatrienoic acids (EETs) such as 5,6-, 8,9-, 11,12-, and 14,15-EETs. In the present study, we analyzed the impact of a genetic variant in CYP2J2 on coronary artery disease (CAD) in the Telangana region of Indian population.
The case-control study consisted of 100 CAD cases and 110 healthy controls. The deoxyribonucleic acid was extracted using the salting out method. Genotyping and gene expression was performed by polymerase chain reaction (PCR)-restriction fragment length polymorphism and real-time-PCR methods.
In the present study, the percentage of smokers, alcoholics, hypertensive patients, and diabetics was high. Increase in fasting glucose, urea, creatinine, fasting triglycerides, total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), total cholesterol/high-density lipoprotein (TC/HDL), LDL/HDL, homocysteine, and C-reactive protein levels were significantly higher in patients with CAD than in controls (p < 0.001). CYP2J2 G-50T was associated with CAD (p = 0.04). The mRNA expression of CYP2J2 showed altered gene expression in this study among CAD patients in comparison with control (p = 0.01).
A functionally relevant polymorphism of the CYP2J2 gene was independently associated with an increased risk of CAD.
细胞色素P-450 2J2（CYP2J2）是已知的肝外组织中花生四烯酸环氧化酶途径的主要酶之一，该途径可产生一系列区域异构体顺式环氧二十碳三烯酸（EETs），如5,6-、8,9-、11,12-和14,15-EETs。在本研究中，我们分析了印度人群特伦甘纳地区CYP2J2基因变异对冠状动脉疾病（CAD）的影响。
病例对照研究包括100例CAD患者和110例健康对照。采用盐析法提取脱氧核糖核酸。通过聚合酶链反应（PCR）-限制性片段长度多态性和实时PCR方法进行基因分型和基因表达分析。
在本研究中，吸烟者、酗酒者、高血压患者和糖尿病患者的比例较高。CAD患者的空腹血糖、尿素、肌酐、空腹甘油三酯、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、总胆固醇/高密度脂蛋白（TC/HDL）、LDL/HDL、同型半胱氨酸和C反应蛋白水平显著高于对照组（p < 0.001）。CYP2J2 G-50T与CAD相关（p = 0.04）。与对照组相比，本研究中CAD患者CYP2J2的mRNA表达显示基因表达改变（p = 0.01）。
CYP2J2基因的一个功能相关多态性与CAD风险增加独立相关。
