High Magnetic Field Laboratory, Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
University of Science and Technology of China, Hefei, Anhui 230036, China.
Oxid Med Cell Longev. 2019 Mar 14;2019:3782074. doi: 10.1155/2019/3782074. eCollection 2019.
Radiotherapy is effective on a large number of cancer types and is one of the most frequently administrated treatments for cancer patients. The anticancer efficacy of X-ray radiotherapy has been frequently correlated with reactive oxygen species (ROS) elevation, which is also a limiting factor for its toxicity on normal tissues. Here, we found that although 4-10 Gy X-rays could significantly reduce cell numbers in both MDA-MB-231 and MCF-7 breast cancer cells, the ROS level changes are less in MCF-7 cells than in MDA-MB-231 cells. Moreover, although both the ROS scavenger N-acetyl-L-cysteine (NAC) and 1 T static magnetic field (SMF) could reduce X-ray-induced ROS elevation, they did not prevent X-ray-induced cell number reduction or cell death increase, which is significantly different from cisplatin. These results demonstrate that although the anticancer efficacy of cisplatin on two breast cancer cell lines is dependent on ROS, the anticancer efficacy of X-ray is not. Moreover, by testing 19 different cell lines, we found that 1 T SMF could effectively reduce ROS levels in multiple cell lines by 10-20%, which encourages further studies to investigate whether SMF could be used as a potential "physical antioxidant" in the future.
放射疗法对大量癌症类型有效，是癌症患者最常接受的治疗方法之一。X 射线放射疗法的抗癌效果经常与活性氧 (ROS) 升高相关联，ROS 升高也是其对正常组织产生毒性的限制因素。在这里，我们发现，尽管 4-10Gy 的 X 射线可以显著减少 MDA-MB-231 和 MCF-7 乳腺癌细胞的数量，但 MCF-7 细胞中的 ROS 水平变化比 MDA-MB-231 细胞中的 ROS 水平变化小。此外，尽管 ROS 清除剂 N-乙酰-L-半胱氨酸 (NAC) 和 1T 静磁场 (SMF) 都可以降低 X 射线诱导的 ROS 升高，但它们并不能预防 X 射线诱导的细胞数量减少或细胞死亡增加，这与顺铂明显不同。这些结果表明，尽管顺铂对两种乳腺癌细胞系的抗癌效果依赖于 ROS，但 X 射线的抗癌效果并非如此。此外，通过测试 19 种不同的细胞系，我们发现 1T SMF 可以有效降低多种细胞系中 10-20%的 ROS 水平，这鼓励进一步研究，以探讨 SMF 是否可以在未来用作潜在的“物理抗氧化剂”。
