Institutes of Physical Science and Information Technology, Anhui University, Hefei 230039, China.
High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.
Cells. 2022 Jan 27;11(3):443. doi: 10.3390/cells11030443.
Although some studies have shown that some static magnetic fields (SMFs) can promote wound healing in diabetic mice, it is not clear whether the other diabetes complications, such as liver disease and diabetic nephropathy, can also be alleviated. Here, we constructed two simple magnetic plates using neodymium permanent magnets to examine the comprehensive effects of moderate SMFs on genetically obese leptin receptor-deficient db/db diabetic mice. We found that although the blood glucose was not obviously reduced by these two SMF settings, both of the glycated serum protein (GSP) and malondialdehyde (MDA) levels were significantly decreased (Cohen's d = 2.57-3.04). Moreover, the wound healing, liver lipid accumulation, and renal defects were all significantly improved by SMF treatment (Cohen's d = 0.91-2.05). Wound tissue examination showed obvious nuclear factor erythroid 2-related factor 2 (NRF2) level decrease (Cohen's d = 2.49-5.40) and Ki-67 level increase (Cohen's d = 2.30-3.40), indicating decreased oxidative stress and increased cell proliferation. In vitro cellular studies with fibroblast NIH3T3 cells showed that SMFs could reduce high glucose-induced NRF2 nucleus translocation (Cohen's d = 0.87-1.15) and cellular reactive oxygen species (ROS) elevation (Cohen's d = 0.92), indicating decreased oxidative stress. Consequently, high glucose-induced impairments in cell vitality, proliferation, and migration were all improved by SMF treatment. Therefore, our results demonstrate that these simple SMF devices could effectively reduce oxidative stress in diabetic mice and may provide a cost-effective physical therapy strategy to alleviate multiple diabetic complications in the future.
虽然一些研究表明，一些静磁场（SMFs）可以促进糖尿病小鼠的伤口愈合，但尚不清楚其他糖尿病并发症，如肝病和糖尿病肾病，是否也可以得到缓解。在这里，我们构建了两个简单的磁板，使用钕永磁体来检查适度 SMF 对遗传性肥胖瘦素受体缺陷 db/db 糖尿病小鼠的综合影响。我们发现，虽然这两种 SMF 设置并没有明显降低血糖，但糖化血清蛋白（GSP）和丙二醛（MDA）水平都明显降低（Cohen's d = 2.57-3.04）。此外，SMF 治疗显著改善了伤口愈合、肝脏脂质积累和肾脏缺陷（Cohen's d = 0.91-2.05）。伤口组织检查显示明显的核因子红细胞 2 相关因子 2（NRF2）水平下降（Cohen's d = 2.49-5.40）和 Ki-67 水平升高（Cohen's d = 2.30-3.40），表明氧化应激减少和细胞增殖增加。体外成纤维细胞 NIH3T3 细胞研究表明，SMF 可以减少高糖诱导的 NRF2 核易位（Cohen's d = 0.87-1.15）和细胞活性氧（ROS）升高（Cohen's d = 0.92），表明氧化应激减少。因此，SMF 治疗改善了高糖诱导的细胞活力、增殖和迁移损伤。因此，我们的结果表明，这些简单的 SMF 装置可以有效地降低糖尿病小鼠的氧化应激，并且可能为未来缓解多种糖尿病并发症提供一种具有成本效益的物理治疗策略。
