Zhang Lei, Ji Xinmiao, Yang Xingxing, Zhang Xin
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui, 230031, P. R. China.
University of Science and Technology of China, Hefei, Anhui, 230036, P. R. China.
Oncotarget. 2017 Feb 21;8(8):13126-13141. doi: 10.18632/oncotarget.14480.
Increasing evidence shows that static magnetic fields (SMFs) can affect cell proliferation but mixed results have been reported. Here we systematically examined the effects of 1 T (Tesla) SMF, which is close to the SMF intensity that patients are exposed to MRI (magnetic resonance imaging) scanners in hospitals, for its effect on 15 different cell lines, including 12 human and 3 rodent cell lines. Our results show that 1 T SMF does not have apparent impact on cell cycle or cell death. However, at higher cell density, it reduced cell numbers in six out of seven solid human cancer cell lines. We found that both cell type and cell density had evident impacts on SMF effects. Moreover, the EGFR-Akt-mTOR pathway, which varies significantly between different cell types and densities, contributes to the differential effects of SMF. In addition, SMF also increases the efficacy of Akt inhibitors on cancer cell growth inhibition. Therefore 1 T SMF affects cell proliferation in a cell type- and cell density-dependent manner, and the inhibition effect of 1 T SMF on multiple cancer cells at higher cell density may indicate its clinical potential in late stage cancer therapy.
越来越多的证据表明，静磁场（SMFs）会影响细胞增殖，但报道的结果不一。在此，我们系统地研究了1特斯拉（T）静磁场的影响，该强度接近患者在医院接受磁共振成像（MRI）扫描时所接触的静磁场强度，研究其对15种不同细胞系的影响，包括12种人类细胞系和3种啮齿动物细胞系。我们的结果表明，1 T静磁场对细胞周期或细胞死亡没有明显影响。然而，在较高细胞密度下，它降低了七种实体人类癌细胞系中六种的细胞数量。我们发现细胞类型和细胞密度对静磁场的影响都有显著作用。此外，表皮生长因子受体-蛋白激酶B-哺乳动物雷帕霉素靶蛋白（EGFR-Akt-mTOR）通路在不同细胞类型和密度之间有显著差异，这导致了静磁场的不同效应。此外，静磁场还增强了蛋白激酶B抑制剂对癌细胞生长的抑制效果。因此，1 T静磁场以细胞类型和细胞密度依赖的方式影响细胞增殖，并且1 T静磁场在较高细胞密度下对多种癌细胞的抑制作用可能表明其在晚期癌症治疗中的临床潜力。
