Liverani Liliana, Killian Manuela S, Boccaccini Aldo R
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
Chair for Surface Science and Corrosion, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
Front Bioeng Biotechnol. 2019 Apr 3;7:68. doi: 10.3389/fbioe.2019.00068. eCollection 2019.
The aim of this study is to demonstrate the feasibility of different functionalization methods for electrospun fibers developed using benign solvents. In particular three different approaches were investigated to achieve the functionalization of poly(epsilon caprolactone) (PCL) electrospun fibers with fibronectin. Protein surface entrapment, chemical functionalization and coaxial electrospinning were performed and compared. Moreover, bilayered scaffolds, with a top patterned and functionalized layer with fibronectin and a randomly oriented not functionalized layer were fabricated, demonstrating the versatility of the use of benign solvents for electrospinning also for the fabrication of complex graded structures. Besides the characterization of the morphology of the obtained scaffolds, ATR-FTIR and ToF-SIMS were used for the surface characterization of the functionalized fibers. Cell adhesion and proliferation were also investigated by using ST-2 cells. Positive results were obtained from all functionalized scaffolds and the most promising results were obtained with bilayered scaffolds, in terms of cells infiltration inside the fibrous structure.
本研究的目的是证明使用良性溶剂制备的电纺纤维采用不同功能化方法的可行性。具体而言，研究了三种不同的方法来实现用纤连蛋白对聚己内酯（PCL）电纺纤维进行功能化。进行并比较了蛋白质表面包埋、化学功能化和同轴电纺。此外，制备了双层支架，其顶层具有纤连蛋白图案化和功能化层，底层为随机取向的非功能化层，证明了良性溶剂用于电纺制备复杂梯度结构的多功能性。除了对所得支架的形态进行表征外，还使用衰减全反射傅里叶变换红外光谱（ATR-FTIR）和飞行时间二次离子质谱（ToF-SIMS）对功能化纤维进行表面表征。还使用ST-2细胞研究了细胞黏附和增殖情况。所有功能化支架均取得了阳性结果，就细胞在纤维结构内的浸润而言，双层支架取得了最有前景的结果。
