Hou Ruolin, Li Lin, Xiang Kaikai, Wu Xiaoping, Lin Wenxiong, Zheng Mingfeng, Fu Junsheng
College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
Sheng Wu Gong Cheng Xue Bao. 2019 Apr 25;35(4):667-676. doi: 10.13345/j.cjb.180353.
Cordyceps militaris exopolysaccharides (EPS) have many pharmacological activities such as boosting immunity and antifatigue. To obtain EPS efficiently, we added moderate Vernonia amygdalina leaf powder as inducer to the fermentation medium to promote the production of Cordyceps militaris EPS and studied the infrared absorption spectrum and antioxidant activities of the EPS after optimization. The optimum liquid fermentation conditions were as follows: addition of Vernonia amygdalina leaf powder of 8 g/L, fermentation duration of 9 d, initial pH of 6.5, inoculation quantity of 5.0 mL. Under such a condition, the yield of Cordyceps militaris EPS reached (5.24±0.28) mg/mL, increased by 205.20% compared to the control group without adding Vernonia amygdalina leaf powder. Results of infrared analysis and antioxidant activity showed that the Vernonia amygdalina leaves had little effect on the structure and activities of Cordyceps militaris EPS. The results of this research suggest that Vernonia amygdalina leaf can enhance the production of Cordyceps militaris EPS effectively, and provides a novel method for efficient production of EPS in Cordyceps militaris.
蛹虫草胞外多糖（EPS）具有多种药理活性，如增强免疫力和抗疲劳。为了高效获得EPS，我们在发酵培养基中添加适量的扁桃斑鸠菊叶粉作为诱导剂，以促进蛹虫草EPS的产生，并在优化后研究了EPS的红外吸收光谱和抗氧化活性。最佳液体发酵条件如下：扁桃斑鸠菊叶粉添加量为8 g/L，发酵时间为9 d，初始pH为6.5，接种量为5.0 mL。在此条件下，蛹虫草EPS产量达到（5.24±0.28）mg/mL，与未添加扁桃斑鸠菊叶粉的对照组相比提高了205.20%。红外分析和抗氧化活性结果表明，扁桃斑鸠菊叶对蛹虫草EPS的结构和活性影响较小。本研究结果表明，扁桃斑鸠菊叶能有效提高蛹虫草EPS的产量，为蛹虫草中EPS的高效生产提供了一种新方法。
