蛹草拟青霉 SU5-08 突变株胞外多糖的发酵生产及其体外抗氧化活性
Production and in vitro antioxidant activity of exopolysaccharide by a mutant, Cordyceps militaris SU5-08.
机构信息
College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
出版信息
Int J Biol Macromol. 2012 Jul-Aug;51(1-2):153-7. doi: 10.1016/j.ijbiomac.2012.04.011. Epub 2012 Apr 20.
Cordyceps militaris SU5-08 was derived from an initial strain (C. militaris SU5) by ultraviolet mutagenesis of protoplasts, and the extraction parameters for C. militaris SU5-08 exopolysaccharide (EPS) produced during submerged culture were optimized. The extraction rate of EPS was 1919.16±165.27 mg/l, which was 120.38±11.36% higher than that of C. militaris SU5. The in vitro scavenging effects of EPS of C. militaris SU5-08 on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals at a dosage of 5 g/l were 63.64±3.52%, 75.27±5.16%, and 6.46±5.03%, respectively. The reducing power of EPS of C. militaris SU5-08 was 0.21±0.01. The results suggest that the EPS of C. militaris SU5-08 can be used as a potential antioxidant which enhances adaptive immune responses.
蛹虫草 SU5-08 是通过原生质体紫外线诱变从初始菌株（蛹虫草 SU5）中衍生而来的，优化了其在液体深层培养中产生的胞外多糖（EPS）的提取参数。EPS 的提取率为 1919.16±165.27mg/L，比蛹虫草 SU5 提高了 120.38±11.36%。在 5g/L 剂量下，蛹虫草 SU5-08 EPS 对羟基自由基、超氧阴离子自由基和 1,1-二苯基-2-苦基肼基（DPPH）自由基的体外清除率分别为 63.64±3.52%、75.27±5.16%和 6.46±5.03%。蛹虫草 SU5-08 EPS 的还原力为 0.21±0.01。结果表明，蛹虫草 SU5-08 EPS 可作为一种潜在的抗氧化剂，增强适应性免疫反应。
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