Esteves Trindade Pedro Henrique, de Camargo Ferraz Guilherme, Pereira Lima Maria Lúcia, Negrão João Alberto, Paranhos da Costa Mateus J R
UNESP, Universidade Estadual Paulista, Programa de Pós-Graduação em Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, São Paulo, Brazil.
UNESP, Universidade Estadual Paulista, Departamento de Morfologia e Fisiologia Animal, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, São Paulo, Brazil.
J Equine Vet Sci. 2019 Apr;75:1-8. doi: 10.1016/j.jevs.2018.11.015. Epub 2018 Dec 21.
This study aimed to investigate the use of maximum eye surface temperature (EST), recorded by infrared thermography, as an indicator of physical fitness in ranch horses. Data collection was carried out with 16 horses by recording six physical fitness biomarkers (EST, respiratory rate, creatine kinase activity [CK], and concentrations of total serum protein, plasmatic cortisol, and lactate) before and after a regular working day. We also recorded heart rate during the exercise, exercise duration, average velocity, distance traveled, and air temperature and humidity. The variation between the sampling times was compared by using repeated measures ANOVA or Wilcoxon paired test. The t-test was used to compare EST means of dehydrated and nondehydrated horses. The differences (DIF-) between each variable's sampling times were calculated, and the Spearman's correlation coefficients between DIF-EST and DIF- of other variables were estimated. A hierarchical cluster analysis was used to classify the horses according to their physical fitness. All biomarkers increased after the workday, and the EST means of dehydrated and nondehydrated horses did not differ (P < .05). DIF-CK and DIF-EST were positively correlated (rs = 0.56, P < .05). The cluster analysis defined two groups of horses (LOW and HIGH), with DIF-EST and DIF-CK differing statistically between them. HIGH horses showed CK higher than 350 U/L, indicating some degree of muscle damage. These results suggest that EST has potential to predict changes in CK and, thus could be a useful indicator of physical fitness in ranch horses; although, its effectiveness has to be confirmed in future studies.
本研究旨在调查通过红外热成像记录的最大眼表温度（EST）作为牧场马匹体能指标的用途。对16匹马进行了数据收集，记录了正常工作日前后的六项体能生物标志物（EST、呼吸频率、肌酸激酶活性[CK]以及血清总蛋白、血浆皮质醇和乳酸的浓度）。我们还记录了运动期间的心率、运动持续时间、平均速度、行进距离以及气温和湿度。使用重复测量方差分析或Wilcoxon配对检验比较采样时间之间的差异。使用t检验比较脱水和未脱水马匹的EST均值。计算每个变量采样时间之间的差异（DIF-），并估计DIF-EST与其他变量的DIF-之间的Spearman相关系数。使用层次聚类分析根据马匹的体能对其进行分类。工作日后所有生物标志物均升高，脱水和未脱水马匹的EST均值无差异（P <.05）。DIF-CK与DIF-EST呈正相关（rs = 0.56，P <.05）。聚类分析定义了两组马匹（低和高），它们之间的DIF-EST和DIF-CK存在统计学差异。高体能组马匹的CK高于350 U/L，表明有一定程度的肌肉损伤。这些结果表明，EST有可能预测CK的变化，因此可能是牧场马匹体能的有用指标；不过，其有效性有待未来研究证实。
