胃癌患者 miR-4317 表达的临床病理及预后意义。

Clinicopathological and prognostic significance of miR-4317 expression in gastric cancer patients.

机构信息

Department of Gastroenterology, Jining No. 1 People's Hospital Affiliated to Jining Medical University, Jining, Shandong, China.

出版信息

Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):2803-2808. doi: 10.26355/eurrev_201904_17555.

DOI:10.26355/eurrev_201904_17555

PMID:31002131

Abstract

OBJECTIVE

Previous studies have revealed that miR-4317 was abnormally expressed and functioned as a tumor suppressor in several tumors, including gastric cancer (GC). However, the clinical significance of miR-4317 in GC remains largely unclear. Our present study aimed at investigating the possibility of miR-4317 as a novel prognostic biomarker for GC patients.

PATIENTS AND METHODS

RT-PCR was performed to determine the levels of miR-4317 in GC tissues and matched normal gastric tissues. Association between miR-4317 levels and clinicopathological factors was also analyzed using Chi-square test. Kaplan-Meier survival analysis and univariate and multivariate assays were further conducted to investigate the correlation between miR-4317 expression and GC patients prognosis.

RESULTS

We showed that miR-4317 expression levels were significantly lower in GC cell lines compared to matched normal tissues (p< 0.01). Down-regulation of miR-4317 was observed to be associated with lymph node metastasis (p = 0.019), distant metastasis (p = 0.011) and TNM stage (p = 0.007). In addition, Kaplan-Meier analysis showed that patients with low miR-4317 expression had a shorter overall survival compared with the high miR-4317 expression group (p = 0.0009). Furthermore, multivariate analysis revealed that miR-4317 expression was an independent prognostic marker of overall survival of GC patients.

CONCLUSIONS

miR-4317 expression may be a novel and valuable prognostic factor in GC.

摘要

目的

先前的研究表明，miR-4317 在多种肿瘤中异常表达并发挥肿瘤抑制作用，包括胃癌（GC）。然而，miR-4317 在 GC 中的临床意义在很大程度上仍不清楚。本研究旨在探讨 miR-4317 作为 GC 患者新型预后生物标志物的可能性。

患者和方法

采用 RT-PCR 检测 GC 组织和配对正常胃组织中 miR-4317 的水平。采用卡方检验分析 miR-4317 水平与临床病理因素的关系。进一步进行 Kaplan-Meier 生存分析和单因素及多因素分析，以探讨 miR-4317 表达与 GC 患者预后的相关性。

结果

我们发现，GC 细胞系中 miR-4317 的表达水平明显低于配对的正常组织（p<0.01）。miR-4317 的下调与淋巴结转移（p=0.019）、远处转移（p=0.011）和 TNM 分期（p=0.007）有关。此外，Kaplan-Meier 分析表明，miR-4317 低表达患者的总生存期明显短于 miR-4317 高表达患者（p=0.0009）。进一步的多因素分析显示，miR-4317 表达是 GC 患者总生存期的独立预后标志物。