Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Aldo Moro, Bari, Italy.
Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):2916-2923. doi: 10.26355/eurrev_201904_17570.
Mesenchymal stem cells (MSCs) have been deeply investigated in regenerative medicine because of their crucial role in tissue healing, such as tissue regeneration. Dental-derived stem cells (d-DSCs) are easily available from dental tissues, which can be isolated from all age patients with minimal discomfort.
Normal unerupted third molars tooth buds were collected from adolescents' patients underwent to extractions for orthodontic reasons. The expression of the genes Kruppel-like factor 4 (Klf-4), octamer-binding transcription factor 4 (Oct-4), homeobox transcription factor Nanog (NANOG) was investigated in d-DSCs obtained from dental bud (DBSCs), differentiated toward osteoblastic phenotype and not.
Our results showed that DBSCs expressed Oct-4, Nanog, and Klf-4 in undifferentiated conditions and interestingly the expression of such genes increased when the cells were kept in osteogenic medium.
These attractive stemness properties, together with the effortlessly isolation, during common oral and maxillofacial surgical procedures, from undifferentiated tissues such as dental bud, make this kind of d-DSCs a promising tool in regenerative medicine, having the potential for clinical applications, and reinforcing the present challenge to develop new preventive and healing strategies in tissue regeneration.
间充质干细胞（MSCs）在再生医学中得到了深入研究，因为它们在组织修复中起着至关重要的作用，例如组织再生。牙源性干细胞（d-DSCs）易于从牙组织中获得，可以从所有年龄段的患者中分离出来，而且几乎没有不适感。
从因正畸原因接受拔牙的青少年患者的未萌出第三磨牙牙胚中收集正常的牙胚。研究了从牙胚中获得的（DBSCs）向成骨表型分化和未分化的 d-DSCs 中 Kruppel 样因子 4（Klf-4）、八聚体结合转录因子 4（Oct-4）、同源盒转录因子 Nanog（NANOG）的基因表达。
我们的结果表明，DBSCs 在未分化状态下表达 Oct-4、Nanog 和 Klf-4，有趣的是，当细胞在成骨培养基中培养时，这些基因的表达增加。
这些有吸引力的干性特征，加上在普通口腔颌面外科手术期间从未分化组织（如牙胚）中轻松分离，使这种 d-DSCs 成为再生医学中一种有前途的工具，具有临床应用的潜力，并加强了目前开发组织再生新的预防和治疗策略的挑战。
