ADIPOQ 基因座 rs1501299 处的单核苷酸多态性与两种低热量饮食中不同类型的膳食脂肪酸相互作用。

Single nucleotide polymorphisms at the ADIPOQ gene locus rs1501299 interact with different type of dietary fatty acids in two hypocaloric diets.

机构信息

Center of Investigation of Endocrinology and Nutrition, School of Medicine, Department of Endocrinology and Nutrition, Hospital Clinico Universitario, University of Valladolid, Valladolid, Spain.

出版信息

Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):2960-2970. doi: 10.26355/eurrev_201904_17577.

DOI:10.26355/eurrev_201904_17577

PMID:31002147

Abstract

OBJECTIVE

Some adiponectin gene (ADIPOQ) and single nucleotide polymorphisms (SNPs) have been related to adiponectin levels and metabolic parameters. Few studies of interaction gene-nutrient have been realized in this topic area. The aim of our study was to analyze the effect of the rs1501299 ADIPOQ gene polymorphism, and the dietary intake on total adiponectin levels and the insulin resistance changes after an enriched-monounsaturated fat (Diet M) vs. an enriched-polyunsaturated fat hypocaloric diet (Diet P).

PATIENTS AND METHODS

A Caucasian population of 363 obese patients was enrolled in a randomized clinical trial with two hypocaloric diets. Before and after 12 weeks on each hypocaloric diet, an anthropometric evaluation, an assessment of nutritional intake and a biochemical analysis were realized. The statistical analysis was performed for the combined GT and TT as a group (mutant) and GG as second group (wild).

RESULTS

With both caloric restriction strategies, body weight, body mass index (BMI), fat mass, waist circumference, systolic blood pressure and leptin levels decreased. After Diet P, only subjects with GG genotype showed a significant improvement in the insulin levels (GG vs. GT±TT) (-3.2±1.0 mU/L vs. -0.6±0.4 mU/L: p=0.01) and in the homeostasis model assessment (HOMA-IR) (-1.1±0.2 units vs. -0.3±0.4 units: p=0.02). The same improvement in both parameters was reported after Diet M: insulin levels (-3.7±0.9 mU/L vs. -0.4±0.5 mU/L: p=0.01) and HOMA-IR (-1.0±0.2 units vs. -0.4±0.3 units: p=0.03). After weight loss with diet M, both genotypes (GG vs. GT±TT) showed similar decrease in total cholesterol and LDL-cholesterol. Only subjects with GG genotype showed a significant increase of the adiponectin levels after both diets: (Diet P: 9.3±3.0 ng/dl vs. Diet M: 8.2±2.9 ng/dl: p=0.38).

CONCLUSIONS

The GG genotype of ADIPOQ gene variant (rs1501299) is associated to a significant improvement in the adiponectin levels and a decrease of insulin and HOMA-IR after two different hypocaloric diets with different profile of unsaturated dietary fats.

摘要

目的

一些脂联素基因（ADIPOQ）和单核苷酸多态性（SNPs）与脂联素水平和代谢参数有关。在这个主题领域，很少有关于基因-营养相互作用的研究。我们的研究目的是分析 rs1501299 脂联素基因多态性和饮食摄入对总脂联素水平的影响，以及富含单不饱和脂肪（饮食 M）与富含多不饱和脂肪低热量饮食（饮食 P）后胰岛素抵抗的变化。

患者和方法

一项纳入 363 名肥胖患者的白种人人群参与了一项随机临床试验，该试验采用两种低热量饮食。在每种低热量饮食 12 周前后，进行了人体测量评估、营养摄入评估和生化分析。对 GT 和 TT 联合作为一组（突变）和 GG 作为第二组（野生）进行了统计分析。

结果

两种热量限制策略均使体重、体重指数（BMI）、体脂、腰围、收缩压和瘦素水平降低。在饮食 P 之后，仅 GG 基因型的受试者显示胰岛素水平（GG 与 GT±TT）显著改善（-3.2±1.0 mU/L 与-0.6±0.4 mU/L：p=0.01）和稳态模型评估（HOMA-IR）（-1.1±0.2 单位与-0.3±0.4 单位：p=0.02）。在饮食 M 后，这两个参数均有相同的改善：胰岛素水平（-3.7±0.9 mU/L 与-0.4±0.5 mU/L：p=0.01）和 HOMA-IR（-1.0±0.2 单位与-0.4±0.3 单位：p=0.03）。在饮食 M 减肥后，两种基因型（GG 与 GT±TT）的总胆固醇和 LDL 胆固醇均有类似的降低。只有 GG 基因型的受试者在两种饮食后脂联素水平显著升高：（饮食 P：9.3±3.0 ng/dl 与饮食 M：8.2±2.9 ng/dl：p=0.38）。