低频超声对颞叶癫痫小鼠的癫痫控制作用。
Seizure control by low-intensity ultrasound in mice with temporal lobe epilepsy.
机构信息
Institute of Electrical Engineering, Yanshan University, Qinhuangdao, 066004, China.
College of Electrical and Control Engineering, North China University of Technology, Beijing, 10041, China.
出版信息
Epilepsy Res. 2019 Aug;154:1-7. doi: 10.1016/j.eplepsyres.2019.04.002. Epub 2019 Apr 4.
BACKGROUND
Temporal lobe epilepsy (TLE) is the most common form of focal epilepsy. Recent studies have demonstrated that ultrasound stimulation can inhibit spontaneous recurrent seizures and improve behavioral outcomes for rodents with TLE. However, the exact underlying mechanism for inhibition of TLE via ultrasound stimulation remains unknown.
METHODS
In this study, low-intensity pulsed ultrasound stimulation (LIPUS) and low-intensity continuous ultrasound stimulation (LICUS) and concurrent local field potentials (LFPs) in the CA3 field of the hippocampus were recorded in the kainite-induced mouse model of TLE. The power spectrum and the phase-amplitude coupling in the LFPs were quantitatively analyzed.
RESULTS
We found three significant changes in LFPs after ultrasound stimulation: (i) the intensity of the power spectrum in the low frequency (<10 Hz) was significantly decreased (p < 0.01); (ii) the phase amplitude coupling strength between slow (delta-, theta-, and alpha-frequency bands) and fast (gamma frequency bands) neural oscillations were weakened (p < 0.01); (iii) the interval between seizures was significantly increased (p < 0.01).
CONCLUSIONS
These results indicate that the seizures of TLE can be effectively inhibited by ultrasound stimulation, and this effect is independent of ultrasound type (pulsed or continuous).
背景
颞叶癫痫（TLE）是最常见的局灶性癫痫形式。最近的研究表明，超声波刺激可以抑制 TLE 自发性复发发作，并改善 TLE 啮齿动物的行为结果。然而，通过超声波刺激抑制 TLE 的确切潜在机制尚不清楚。
方法
在这项研究中，在海人酸诱导的 TLE 小鼠模型中记录了低强度脉冲超声波刺激（LIPUS）和低强度连续超声波刺激（LICUS）以及海马 CA3 区的局部场电位（LFPs）。对 LFPs 中的功率谱和相位-幅度耦合进行了定量分析。
结果
我们发现超声波刺激后 LFPs 有三个明显变化：（i）低频（<10 Hz）的功率谱强度显着降低（p < 0.01）；（ii）慢（δ、θ和α频带）和快（γ频带）神经振荡之间的相位幅度耦合强度减弱（p < 0.01）；（iii）发作之间的间隔显着增加（p < 0.01）。
结论
这些结果表明，超声波刺激可以有效抑制 TLE 的发作，并且这种效果与超声波类型（脉冲或连续）无关。
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