3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's PT Associate Laboratory, Guimarães, Portugal.
ICVS/3B's PT Associate Laboratory, Guimarães, Portugal; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.
Int J Biol Macromol. 2019 Jul 15;133:933-944. doi: 10.1016/j.ijbiomac.2019.04.102. Epub 2019 Apr 16.
The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic. These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-l-alanine (DOPA), which presents the reactive catechol group. Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties. In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films. Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed. In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer. Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect. Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.
海洋贻贝类蛋白质的黏附能力是众所周知的，它能够黏附在不同的水下基质上，无论是无机的还是有机的。这些独特的黏附特性归因于高含量的氨基酸，即 3,4-二羟基苯丙氨酸（DOPA），它具有反应性的儿茶酚基团。在此，开发了具有天然聚合物壳聚糖（CHT）和透明质酸（HA）的新型抗菌独立式（FS）薄膜，其中还包括儿茶酚功能化透明质酸（HA-DN）以提供湿黏附特性。为了获得复合薄膜，掺入了银掺杂生物玻璃纳米粒子（Ag-BGs）以促进杀菌和生物活性，测试了四种不同配方的 FS 薄膜。分析了它们的表面形貌和拓扑结构、润湿性、失重、溶胀、机械性能、黏附性和生物活性。特别是，生物活性测试表明，在模拟体液中浸泡后，会形成类似骨的磷灰石层。此外，在与金黄色葡萄球菌和大肠杆菌培养物直接接触 16 小时后，这些 FS 薄膜表现出明显的抗菌作用。因此，这种具有生物活性、抗菌和黏附性的独立式薄膜可能被用作临时引导骨再生膜，特别是用于再生小的骨缺损和牙周组织。
