Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province, 650500, China; Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Yunnan Province, 650500, China.
College of Basic Medical Sciences, Shenyang Medical College, Liaoning Province, 110000, China.
Ecotoxicol Environ Saf. 2019 Aug 30;178:130-136. doi: 10.1016/j.ecoenv.2019.04.021. Epub 2019 Apr 16.
A simple, effective and convenient method for determination of phenolic compounds by acid-base induced deep eutectic solvents (DESs) microextraction was developed. The binary and ternary DESs were prepared by a range of fatty acids (C-C), which can act as hydrogen bond donors and hydrogen bond acceptors simultaneously. The gas-assisted mixing customization provides excellent mixing performance and concentration efficiency through the bubble adsorption mechanism for the handling of large-volume aqueous sample. In extraction process, NH·HO can act as the emulsifier agent and reacted with DESs to form salts with a cloudy solution, which can obviously improve the extraction efficiency. HCl can act as the phase separation agent, and there is no need to centrifuge, which increases the efficiency of analysis procedure. The factors affected on extraction efficiency were carefully optimized. At optimum conditions and molar ratio of C:C:C (3:2:1), the limit of detections (LODs), the preconcentration factor, the repeatability (RSDs%) were in the range of 0.22-0.53 μg L, 235-244, and 2.6-6.7%, respectively. Finally, the proposed method was applied to analyze four phenolic compounds in real water samples and the recoveries were between 87.4% and 106.6%.
建立了一种基于酸碱诱导的深共晶溶剂（DESs）微萃取测定酚类化合物的简单、有效、便捷的方法。二元和三元 DESs 由一系列脂肪酸（C-C）组成，这些脂肪酸可以同时充当氢键供体和氢键受体。气体辅助混合定制通过气泡吸附机制提供了出色的混合性能和浓度效率，可用于处理大容量水相样品。在萃取过程中，NH·HO 可以作为乳化剂与 DESs 反应形成混浊溶液的盐，这可以明显提高萃取效率。HCl 可以作为相分离剂，无需离心，从而提高了分析程序的效率。仔细优化了影响萃取效率的因素。在最佳条件和 C:C:C（3:2:1）摩尔比下，检测限（LOD）、预浓缩因子和重复性（RSDs%）的范围分别为 0.22-0.53μg/L、235-244 和 2.6-6.7%。最后，该方法用于分析实际水样中的四种酚类化合物，回收率在 87.4%至 106.6%之间。
