茯苓多糖通过巨噬细胞中的 Ca/PKC/p38/NF-κB 信号通路发挥免疫调节作用。
The immunomodulatory effect of Poria cocos polysaccharides is mediated by the Ca/PKC/p38/NF-κB signaling pathway in macrophages.
机构信息
Department of Clinical Laboratory, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
The Second Clinic College, Chongqing Medical University, Chongqing 400010, China.
出版信息
Int Immunopharmacol. 2019 Jul;72:252-257. doi: 10.1016/j.intimp.2019.04.017. Epub 2019 Apr 16.
Poria cocos polysaccharide (PCP), extracted from Poria cocos sclerotium, has many biological activities. The present study explored the immunomodulatory effect and the underlying molecular mechanism of PCP in RAW 264.7 macrophages. Griess reaction, ELISA assays and confocal laser scanning microscopy revealed that the production of nitric oxide (NO), TNF-α, IL-1β, IL-6 and intracellular calcium level were increased by PCP. However, this effect on cytokines was suppressed with a Ca channel blocker or a p38 inhibitor, which indicates that Ca and p38 are crucial to the immunomodulatory effect of PCP. We further demonstrated that PCP-treated cells also exhibited increased the activity of PKC, mRNA and protein expression levels of p38 and NF-κB, which is also reduced with a Ca channel blocker. Taken together, the Ca/PKC/p38/NF-κB signaling pathway may involve in the immunomodulatory effects of PCP.
茯苓多糖（PCP）从茯苓菌核中提取，具有多种生物活性。本研究探讨了 PCP 在 RAW264.7 巨噬细胞中的免疫调节作用及其潜在的分子机制。格里斯反应、ELISA 检测和共聚焦激光扫描显微镜显示，PCP 增加了一氧化氮（NO）、TNF-α、IL-1β、IL-6 和细胞内钙离子水平。然而，用钙通道阻滞剂或 p38 抑制剂抑制了这种细胞因子的产生，这表明钙和 p38 对 PCP 的免疫调节作用至关重要。我们进一步证明，PCP 处理的细胞还表现出蛋白激酶 C（PKC）活性增加，p38 和 NF-κB 的 mRNA 和蛋白表达水平也降低，这也可以用钙通道阻滞剂来减少。综上所述，钙/蛋白激酶 C/p38/NF-κB 信号通路可能参与 PCP 的免疫调节作用。
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