Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China.
Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jul 5;218:243-247. doi: 10.1016/j.saa.2019.04.020. Epub 2019 Apr 11.
Osteoarthritis (OA) is not only related to the degradation of articular cartilage, but also possibly to the changes of subchondral bone. The purpose of this study was to assess whether specific differences could be resolved from bone composition, as also contributed to OA. These differences were assessed by using Fourier transform infrared spectroscopy (FTIRS). The main parameters including mineral content, carbonate content, crystallinity, collagen cross-linking ratio (XLR) and acid phosphate content were represented with characteristic peak integration. It was found that mineral and carbonate content varied significantly with depths at different OA stages. Mineral content increased with depth in healthy samples, while carbonate content showed opposite trend. The mineral content reduced obviously with OA duration, which was different with carbonate decreasing only at early stage of OA. In addition, the content of acid phosphate, collagen maturity (XLR) and crystallinity slight varied with the OA aggravation. Therefore, the changes in subchondral bone were significantly associated with cartilage degeneration and OA, the associated parameters should be targeted for OA therapies.
骨关节炎（OA）不仅与关节软骨的降解有关，还可能与软骨下骨的变化有关。本研究旨在通过傅里叶变换红外光谱（FTIRS）评估是否可以从骨成分中分辨出导致 OA 的特定差异。这些差异通过特征峰积分来表示，包括矿物质含量、碳酸盐含量、结晶度、胶原交联比（XLR）和酸性磷酸盐含量。研究发现，不同 OA 阶段的不同深度的矿物质和碳酸盐含量有显著差异。健康样本中矿物质含量随深度增加而增加，而碳酸盐含量则呈现相反的趋势。随着 OA 持续时间的延长，矿物质含量明显下降，而碳酸盐含量仅在 OA 的早期阶段下降。此外，酸性磷酸盐、胶原成熟度（XLR）和结晶度的含量也随 OA 的加重而略有变化。因此，软骨下骨的变化与软骨退变和 OA 密切相关，OA 治疗应针对相关参数。
