Fouet A, Klier A, Rapoport G
Gene. 1986;45(2):221-5. doi: 10.1016/0378-1119(86)90258-1.
The sucrase gene (sacA) and part of the sacP locus, which corresponds to a membrane component of the phosphotransferase system (PTS) of sucrose transport of Bacillus subtilis, were previously cloned on a 2.1-kb EcoRI DNA fragment. Genes sacA and sacP were localized on this DNA fragment and the nucleotide sequence of the 2.1-kb DNA fragment was determined. A 1440-bp open reading frame (480 codons) was identified coding for a deduced polypeptide of Mr54827, which corresponds to that of purified sucrase. The amino acid sequence shares homology with that of yeast invertase (SUC2 gene product). The sacA gene and the preceding sacP gene seem to belong to the same operon.
蔗糖酶基因（sacA）和sacP基因座的一部分，该部分对应于枯草芽孢杆菌蔗糖转运磷酸转移酶系统（PTS）的一个膜组分，先前已克隆到一个2.1kb的EcoRI DNA片段上。sacA基因和sacP基因定位在该DNA片段上，并测定了2.1kb DNA片段的核苷酸序列。鉴定出一个1440bp的开放阅读框（480个密码子），编码一个推导的Mr54827多肽，其与纯化的蔗糖酶相对应。该氨基酸序列与酵母转化酶（SUC2基因产物）的氨基酸序列具有同源性。sacA基因和其前面的sacP基因似乎属于同一个操纵子。
