Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Deparment of Medical Laser, Medical Laser Research Center, YARA Institute, ACECR, Tehran, Iran.
J Biochem Mol Toxicol. 2019 Jul;33(7):e22325. doi: 10.1002/jbt.22325. Epub 2019 Apr 20.
Cartap hydrochloride is a mildly perilous insecticide known as "Padan" which is used largely in agricultural farms to control weevil and caterpillars. The over use of cartap causes harmful effects on human health. Since the blood may acts as a target and carrier for insecticides, the effect of these compounds on blood in mammalian toxicology is very important. Hemoglobin is a tetramer protein that play critical role in oxygen transport. The aim of this study was to analyze and compare the function and structural changes of hemoglobin in the presence of different concentrations of cartap by employing different spectroscopic techniques. The obtained results show that cartap has a high hemolytic effect which is increased with cartap concentration and reduces the thermal midpoint of hemoglobin. Fluorescence measurements reveal heme degradation at different concentrations of cartap. In consequence of theoretical and experimental results, cartap has an undesirable effect on hemoglobin structure and function.
盐酸杀虫脒是一种低毒性杀虫剂，俗称“帕丹”，主要用于农业农场防治象鼻虫和毛毛虫。过度使用杀虫脒会对人体健康造成有害影响。由于血液可能是杀虫剂的靶标和载体，因此这些化合物对哺乳动物毒理学中血液的影响非常重要。血红蛋白是一种四聚体蛋白，在氧气运输中起着关键作用。本研究的目的是通过使用不同的光谱技术分析和比较在不同浓度的杀虫脒存在下血红蛋白的功能和结构变化。实验结果表明，杀虫脒具有很高的溶血作用，其溶血作用随杀虫脒浓度的增加而增加，并降低血红蛋白的热中点。荧光测量表明在不同浓度的杀虫脒存在下，血红素发生降解。根据理论和实验结果，杀虫脒对血红蛋白的结构和功能有不良影响。
