研究羧甲基普鲁兰聚糖对兔眼免受系统诱导的角膜前泪膜损伤的保护作用。

Investigating the potential of carboxymethyl pullulan for protecting the rabbit eye from systematically induced precorneal tear film damage.

机构信息

Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, 147 002, Punjab, India.

Pharmaceutics Division, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147 002, Punjab, India.

出版信息

Exp Eye Res. 2019 Jul;184:91-100. doi: 10.1016/j.exer.2019.04.017. Epub 2019 Apr 17.

DOI:10.1016/j.exer.2019.04.017

PMID:31004572

Abstract

The present investigation was aimed to develop a rabbit model for protecting the rabbit eye from systematically induced precorneal tear film (PTF) damage, evaluation of carboxymethyl pullulan for its protective action against PTF damage and its curative potential. For the same, pullulan was modified by carboxymethylation and structural modification was confirmed by spectral attributes. Further, the carboxymethyl pullulan (CMP) solutions (0.1-2.0%, w/v) were evaluated for their physical properties and its concentration 1.5% (w/v) was found to fit the criterion to prepare an eye solution. The safety and non-toxicity of CMP (1.5%, w/v) eye solution was confirmed by HET-CAM method and rabbit eye irritation test. Further, a systematic rabbit eye model was developed that mimic PTF damage in day to day life. Therefore, three levels of PTF damage were developed equating symptoms of damage due to high temperature (level I) or long term mobile use (level II) or heavy air pollution (level III). Thus, a representative model with benzalkonium chloride (BAC, 0.1% v/v, 0.2% v/v and 0.3% v/v), administered two drops twice a day for two days to develop level I, level II and level III eye damage. The CMP (1.5%, w/v) eye solution possessed a protective potential against level I and II PTF damage. The rabbit eyes remained unharmed and comparable with the normal control during the complete experimental period. Additionally, CMP (1.5%, w/v) eye solution has shown early fast recovery (8 days) from PTF damage induced by instillation of PTF damage agent (BAC). Carboxymethyl pullulan eye protective solution has normalized the tear film stability in rabbit eye model. It is established from the present work that, carboxymethyl pullulan has protective action against precorneal tear film damage and it potentiates the early recovery too.

摘要

本研究旨在建立一种保护兔眼免受系统诱导的预角膜泪膜（PTF）损伤的模型，评价羧甲基普鲁兰聚糖对 PTF 损伤的保护作用及其治疗潜力。为此，通过羧甲基化对普鲁兰聚糖进行修饰，并通过光谱属性确认结构修饰。此外，评估了羧甲基普鲁兰聚糖（CMP）溶液（0.1-2.0%，w/v）的物理性质，发现其 1.5%（w/v）浓度符合制备眼用溶液的标准。通过 HET-CAM 法和兔眼刺激性试验证实 CMP（1.5%，w/v）眼用溶液的安全性和非毒性。进一步，建立了一种系统的兔眼模型，模拟日常生活中的 PTF 损伤。因此，开发了三种 PTF 损伤水平，使因高温（水平 I）或长期使用移动设备（水平 II）或重度空气污染（水平 III）引起的损伤症状相匹配。因此，建立了一个代表性模型，用苯扎氯铵（BAC），0.1%v/v、0.2%v/v 和 0.3%v/v，每天两次滴两滴，持续两天，以开发水平 I、水平 II 和水平 III 眼部损伤。CMP（1.5%，w/v）眼用溶液对水平 I 和 II PTF 损伤具有保护作用。在整个实验期间，兔眼保持完好无损，与正常对照组相当。此外，CMP（1.5%，w/v）眼用溶液在滴入 PTF 损伤剂（BAC）引起 PTF 损伤后显示出早期快速恢复（8 天）。羧甲基普鲁兰聚糖眼保护溶液可使兔眼模型中的泪膜稳定性正常化。从本工作中可以得出结论，羧甲基普鲁兰聚糖对预角膜泪膜损伤具有保护作用，并能促进早期恢复。