Del Fabbro Lucian, Jesse Cristiano Ricardo, de Gomes Marcelo Gomes, Borges Filho Carlos, Donato Franciele, Souza Leandro Cattelan, Goes André Rossito, Furian Ana Flávia, Boeira Silvana Peterini
Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas (Laftambio Pampa), Universidade Federal Do Pampa, Campus Itaqui, 97650-000, Itaqui, RS, Brazil.
Laboratório de Farmacologia, Micotoxinas e Toxicologia (Lafarmt), Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
Toxicon. 2019 Jul;165:13-21. doi: 10.1016/j.toxicon.2019.04.004. Epub 2019 Apr 17.
The mycotoxin zearalenone (ZEA) has strong estrogenic effects and elicits reproductive toxicity. Chrysin is a natural flavonoid found in many plant and has a broad range of pharmacological activities, including anticancer, antioxidant and anti-inflammatory. The present study aimed to investigate the potential protective effects of chrysin against ZEA toxicity. Mice received chrysin (5 or 20 mg/kg; i.g.) for ten days, and then received a single injection of ZEA (40 mg/kg). Two days thereafter, blood and testes were collected. ZEA decreased number and motility of sperm, plasma testosterone levels, enzymatic (glutathione peroxidase, glutathione reductase, glutathione-S-transferase) and non-enzimatic defenses (reduced glutathione). Moreover, ZEA increased 4-hydroxynonenal and 8-hydroxy-2'-deoxyguanosine levels, myeloperoxidase activity and levels of proinflammatory cytokines (interleukins-1β and 6, tumor necrosis factor alpha). ZEA also decreased levels of anti-inflammatory cytokine interleukin-10 and increased activity of caspases 3 and 9. Chrysin treatment increased the number and motility of sperm, testosterone levels, restored antioxidant defenses and reduced the inflammation and apoptosis process. In summary, chrysin attenuated the toxic effects caused by ZEA in blood and testes of mice, suggesting a potential preventive treatment against the deleterious effects of ZEA.
霉菌毒素玉米赤霉烯酮（ZEA）具有很强的雌激素效应并引发生殖毒性。白杨素是一种存在于许多植物中的天然黄酮类化合物，具有广泛的药理活性，包括抗癌、抗氧化和抗炎作用。本研究旨在探讨白杨素对ZEA毒性的潜在保护作用。小鼠连续十天接受白杨素（5或20毫克/千克；腹腔注射），然后单次注射ZEA（40毫克/千克）。此后两天，采集血液和睾丸。ZEA降低了精子数量和活力、血浆睾酮水平、酶促防御（谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽-S-转移酶）和非酶促防御（还原型谷胱甘肽）。此外，ZEA增加了4-羟基壬烯醛和8-羟基-2'-脱氧鸟苷水平、髓过氧化物酶活性以及促炎细胞因子（白细胞介素-1β和6、肿瘤坏死因子α）的水平。ZEA还降低了抗炎细胞因子白细胞介素-10的水平，并增加了半胱天冬酶3和9的活性。白杨素治疗增加了精子数量和活力、睾酮水平，恢复了抗氧化防御能力，并减轻了炎症和凋亡过程。总之，白杨素减轻了ZEA对小鼠血液和睾丸造成的毒性作用，表明其对ZEA的有害影响具有潜在的预防治疗作用。
